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Published June 2013 | Supplemental Material + Accepted Version
Journal Article Open

Photothermal bleaching in time‐lapse photoacoustic microscopy


We studied the phenomenon of photothermal bleaching – a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy, pulse duration, and the absorber's size was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time‐lapse imaging applications.

Additional Information

© 2013 WILEY‐VCH. Manuscript received: 11 September 2012. Manuscript revised: 23 October 2012. Manuscript accepted: 25 October 2012. Version of Record online: 26 November 2012. Special Issue: Optoacoustic Imaging and Spectroscopy for Biomedical Diagnosis. This work was sponsored by the National Institutes of Health (NIH) under grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L. V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc.; however, neither provided support for this work.

Attached Files

Accepted Version - nihms417528.pdf

Supplemental Material - jbio_201200184_sm_author_biographies.pdf


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