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Published March 1988 | metadata_only
Journal Article

Conserved and Variable Regions in the Subunits of Brain Type II Ca^(2+)/Calmodulin-Dependent Protein Kinase


Brain type II Ca2+/calmodulin-dependent protein kinase is a holoenzyme composed of several copies each of three subunits, alpha (50 kd), beta (60 kd), and beta' (58 kd), in varying proportions. The deduced amino acid sequences of alpha (reported here) and beta are highly similar but not identical. The major difference between them is the deletion from alpha of two short segments (residues 316-339 and 354-392 in beta). cDNAs that appear to encode beta' are identical to beta except for the deletion of a segment encoding residues 378-392. Thus, the structural differences among alpha, beta, and beta' arise primarily from deletions (or insertions) in a variable region lying immediately carboxyl to the protein kinase and calmodulin-binding domains. The alpha and beta subunits are encoded by distinct genes expressed primarily, if not exclusively, in brain. Rather than being encoded by a third gene, beta' may arise by alternative splicing of the beta gene transcript.

Additional Information

Elsevier Copyright © 1988. Received 30 October 1987. Revised 18 December 1987. We thank N. Qian and T. j. Sejnowski for providing us with the predictions of secondary structure, G. Schull for providing a rat brain cDNA library, Norman Davidson and Judith Campbell for helpful comments on the manuscript, and C. Hochenedel for help with preparation of the manuscript. This work was supported by National Institutes of Health Grant NS 17660 to M. B. K., training grants NS 07251 and GM 07737, the McKnight Foundation, and the Gustavus and Louise Pfeiffer Research Foundation.

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August 19, 2023
August 19, 2023