Anatomical and metabolic small-animal whole-body imaging using ring-shaped confocal photoacoustic computed tomography
Due to the wide use of animals for human disease studies, small animal whole-body imaging plays an increasingly important role in biomedical research. Currently, none of the existing imaging modalities can provide both anatomical and glucose metabolic information, leading to higher costs of building dual-modality systems. Even with image coregistration, the spatial resolution of the metabolic imaging modality is not improved. We present a ring-shaped confocal photoacoustic computed tomography (RC-PACT) system that can provide both assessments in a single modality. Utilizing the novel design of confocal full-ring light delivery and ultrasound transducer array detection, RC-PACT provides full-view cross-sectional imaging with high spatial resolution. Scanning along the orthogonal direction provides three-dimensional imaging. While the mouse anatomy was imaged with endogenous hemoglobin contrast, the glucose metabolism was imaged with a near-infrared dye-labeled 2-deoxyglucose. Through mouse tumor models, we demonstrate that RC-PACT may be a paradigm shifting imaging method for preclinical research.
Additional Information© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE). The authors appreciate Ms. Seema Dahlheimer's close reading of the manuscript. This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, R01 CA157277, R01 CA159959, and 5P60 DK02057933. L.W. has financial interests in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
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