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Draft Genome Sequence of
Ardenticatena maritima
110S, a
Thermophilic Nitrate- and Iron-Reducing Member of the
Chloroflexi
Class
Ardenticatenia
James Hemp,
a
Lewis M. Ward,
a
Laura A. Pace,
b
Woodward W. Fischer
a
Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA
a
; Department of Medicine, University of California San Diego,
La Jolla, California, USA
b
We report here the draft genome sequence of
Ardenticatena maritima
110S, the first sequenced member of class
Ardenticatenia
of the phylum
Chloroflexi
. This thermophilic organism is capable of a range of physiologies, including aerobic respiration and
iron reduction. It also encodes a complete denitrification pathway with a novel nitric oxide reductase.
Received
28 September 2015
Accepted
5 October 2015
Published
19 November 2015
Citation
Hemp J, Ward LM, Pace LA, Fischer WW. 2015. Draft genome sequence of
Ardenticatena maritima
110S, a thermophilic nitrate- and iron-reducing member of the
Chloroflexi
class
Ardenticatenia
. Genome Announc 3(6):e01347-15. doi:10.1128/genomeA.01347-15.
Copyright
© 2015 Hemp et al. This is an open-access article distributed under the terms of the
Creative Commons Attribution 3.0 Unported license
.
Address correspondence to James Hemp, jimhemp@caltech.edu.
A
rdenticatena maritima
110S was originally isolated from an
iron-rich coastal hydrothermal field in the Kirishima Volca-
nic Belt of Japan (
1
). Closely related strains have been reported
from hot springs (
2
) and hydrothermal vents (
3
).
A. maritima
is a
filamentous nonmotile organism that can facultatively reduce ni-
trate and iron (
1
). It grows optimally at 50 to 70°C and pH 7.0
(pH range, 5.5 to 8.0).
The genome of
A. maritima
110S (DSM 23922) was sequenced
as part of a project to expand the phylogenetic breadth of
Chloro-
flexi
genomes. Genome sequencing was performed at SeqMatic
using the Illumina MiSeq sequencing platform. SPAdes 3.1.1 (
4
)
was used to assemble the genome. The genome was screened for con-
taminants based on sequence coverage, G

C composition, and
BLAST hits of conserved single-copy genes. Genome annotation was
performed using the NCBI Prokaryotic Genome Annotation Pipe-
line. The draft genome is 3.62 Mb in size, assembled into 12 contigs. It
contains 3,041 genes, 2,578 coding sequences (CDSs), 2 16S RNAs, 46
tRNAs, and 10 clustered regularly interspaced short palindromic re-
peat (CRISPR) arrays. It is estimated to be ~99% (111/111) complete
based on conserved single-copy genes.
Analysis of the
A. maritima
genome revealed the presence of
many genes responsible for its physiological breadth
. A. maritima
encodes a branched aerobic respiratory chain, including complex
I (NADH dehydrogenase), complex II (succinate dehydrogenase),
complex III (cytochrome
bc
complex), and three oxygen reductases
(A- and B-family heme-copper oxygen reductases and
bd
oxidase).
B-family heme-copper oxygen reductases are commonly found in
aerobic thermophiles, enabling growth with the low oxygen levels
found in thermal systems (
5
).
A. maritima
also encodes a complete
denitrification pathway composed of nitrate reductase (NapA), ni-
trite reductase (NirK), a novel nitric oxide reductase (eNOR) (
6
), and
nitrous oxide reductase (NosZ). Interestingly, the eNOR is found in
an operon with NirK, suggesting that these genes are coregulated. No
genes were found for either lipopolysaccharide (LPS) biosynthesis or
outer membrane proteins, consistent with the proposal that
Chloro-
flexi
have only one membrane (
7
).
Nucleotide sequence accession number.
This whole-genome
shotgun project has been deposited in DDBJ/EMBL/GenBank un-
der the accession no.
LGKN00000000
.
ACKNOWLEDGMENTS
Genomic DNA was obtained from the Leibniz-Institut DSMZ-Deutsche
Sammlung von Mikroorganismen und Zellkulturen GmbH. Sequencing
was performed at SeqMatic, Fremont, CA.
This work was funded in part by the Center for Environmental Micro-
bial Interactions (CEMI) at Caltech, the Packard Foundation (to
W.W.F.), the Agouron Institute (to J.H. and W.W.F.), and NSF GRFP (to
L.M.W.).
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