1
Supporting information
Nanomotor
-
based Biocatalytic Patterning of Helical
Metal Microstructures
Kalayil Manian Manesh, Susana Campuzano, Wei Gao, Mar
í
a Jes
ú
s Lobo
-
Castañ
ó
n
,
Isao
Shitanda, Kiarash Kiantaj, Joseph Wang*
Department of Nanoengineering,
University of California San Diego,
La Jolla, CA 92093, USA
*E
-
mail:
josephwang@ucsd.edu
TABLE OF CONTENTS
SI Video 1
SI Figure 1
Residual GO
x
enzymatic activity remained after membrane dissolution
Electronic Supplementary Material (ESI) for Nanoscale
This journal is © The Royal Society of Chemistry 2012
2
SI Video 1.
Movement
of
a
flexible
GOx
-
Au/Ag
Flex
/Ni nanowire in
the developing
solution containing
10 mM glucose and 0.3 mM AuCl
4
-
.
Conditions, as in Figure 1.
SI Figure 1.
Schematic representation of the surface chemistry involved in the magnetic
swimmer modification with GOx.
Residual GO
x
enzymatic activity remained after membrane dissolution
In order to corroborate that the enzymatic activity of the GO
x
immobilized on th
e
nanomotors survived the harsh conditions during membrane dissolution we performed
additional electrochemical experiments by immobilizing the enzyme, using the same
protocol followed for b
io
-
functionalization of the nanowires
, on
Au/SPEs
.
These
Au/SPEs
in
clude a
Ag
pseudoreference
electrode
and
a
Au
counter electrode
.
After
immobilization of the enzyme, the electrodes were washed and dried and a
10
L
drop
of
a 0.3 M NaOH
solution
was
placed on the GO
x
-
modified Au/SPEs surface
and incubated
during
24 h at
4 ºC in
an
humidified
chamber
.
After washing and drying
c
hronoamperometric
detection was performed
by
placing a 50
L drop of a mixture
containing 1 mM TMB, 1 mM glucose and 136.4 U mL
-
1
HRP
(
in phosphate buffer 0.1
Electronic Supplementary Material (ESI) for Nanoscale
This journal is © The Royal Society of Chemistry 2012
3
M
pH 7.4)
on the
sensor,
stepp
ing the
potential
to
-
200 mV (vs the
Ag
pseudoreference
electrode), and sampl
ing
the
resulting
current at
different times
.
By comparison of the current values obtained over GO
x
-
modified Au/SPEs
exposed or not to the 0.3 M NaOH solution allows to conclude that the
enzyme
retained
approximately the 23.5
%
(mean value of 5 independent experiments)
of its original
activity after its exposure to the harsh basic conditions required for dissolving the
membrane.
The obtained results are in agreement with the reported survi
val of HRP also
to these harsh conditions.
2
2
Electronic Supplementary Material (ESI) for Nanoscale
This journal is © The Royal Society of Chemistry 2012