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Published November 15, 2015 | public
Journal Article Open

High resolution data-independent acquisition with electron transfer dissociation mass spectrometry: Multiplexed analysis of post-translationally modified proteins

Abstract

Data-dependent acquisition (DDA) mode is the most commonly used method in bottom-up proteomics. Recently, data-independent acquisition (DIA) modes have become popular alternatives because of their unbiased analysis, leading in general to more comprehensive, global qualitative profiling of proteome systems and also higher quantitative reproducibility in such profiling. Most of the previously established DIA methods are based on collision-induced dissociation (CID). However, when it comes to the analysis of labile post-translational modifications (PTMs), electron capture/transfer dissociation (ECD/ETD) may be better suited. In addition to the bottom-up approach, the middle-down approach, which analyzes peptides in the range of 3,000–10,000 Da has emerged as an attractive alternative, including the analysis of highly modified and highly variable protein variants that exist in key system functions, such as histone signaling cascades. Here, we establish that a data-independent (DIA) middle-down ETD approach is a superior strategy in the differential characterization of PTM changes in histone H2B. We suggest that this strategy can further be used for other approaches where dynamic PTM characterization or changes due to different conditions are fundamental to accurate understanding of biological systems and function.

Additional Information

© 2015 Elsevier B.V. Received 15 April 2015; Received in revised form 24 June 2015; Accepted 29 June 2015; Available online 20 July 2015. This work was supported by the Betty and Gordon Moore Foundation (GBMF775), the Beckman Institute, and HHMI (through the acquisition of the Orbitrap Fusion). Conflict of interest statement: TPS is an employee of Thermo Fisher Scientific.

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