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Published October 5, 2015 | public
Journal Article Open

Vectored antibody gene delivery mediates long-term contraception


Development of non-surgical methods of long-term or permanent contraception remains a challenge. Towards this objective, we show that intramuscular injection of a replication-incompetent, recombinant adeno-associated virus (rAAV) designed to express an antibody that binds gonadotropin-releasing hormone (GnRH), a master regulator of reproduction in vertebrates, results in long-term infertility in male and female mice. Female mice are also rendered infertile through rAAV-dependent expression of an antibody that binds to the zona pellucida (ZP), a glycoprotein matrix that surrounds the egg and functions as a sperm-binding site. Many proteins known or suspected to be important for reproduction can be targeted, potentially reversibly, using this approach, which we refer to as vectored contraception (VC).

Additional Information

© 2015 Elsevier Ltd. We thank Janet Baer and Karen Lencioni for providing advice on a number of aspects of this work relating to mouse husbandry and reproduction. Shirley Pease provided help with mouse superovulation and Ken Chan with microscopy of eggs. Alejandro B. Balazs and Rachel Galimidi provided help with AAV. Jost Vielmetter and the Caltech Protein Expression Center provided assistance with protein production and SPR measurements. Cynthia Besch-Williford (IDEXX Bioresearch) provided analysis of mouse histology. This work did not receive specific funding. The Proteome Exploration Laboratory (A.M., M.J.S., S.H.) was supported by the Gordon and Betty Moore Foundation through Grant GBMF775, the Beckman Institute, and the NIH, through Grant 1S10RR029594-01A1. A. Olvera was supported by a Gates Millennium Scholars award.

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August 20, 2023
August 20, 2023