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Published May 2011 | Published + Supplemental Material
Journal Article Open

Synergistic actions between the SRP RNA and translating ribosome allow efficient delivery of the correct cargos during cotranslational protein targeting


During cotranslational protein targeting by the Signal Recognition Particle (SRP), the correct cargo accelerates stable complex assembly between the SRP and SRP receptor (FtsY) by several orders of magnitude, thus enabling rapid and faithful cargo delivery to the target membrane. The molecular mechanism underlying this cargo-induced rate acceleration has been unclear. Here we show that the SRP RNA allows assembly of the SRP–FtsY complex to be specifically stimulated by a correct cargo, and, reciprocally, a correct cargo enables the SRP RNA to optimize its electrostatic interactions with FtsY. These results combined with recent structural work led us to suggest a "conformational selection" model that explains the synergistic action of the SRP RNA with the cargo in accelerating complex assembly. In addition to its previously proposed role in preventing the premature dissociation of SRP and FtsY, we found that the SRP RNA also plays an active role in ensuring the formation of productive assembly intermediates, thus guiding the SRP and FtsY through the most efficient pathway of assembly.

Additional Information

© 2011 RNA Society. Published by Cold Spring Harbor Laboratory Press. Received December 21, 2010; accepted February 22, 2011. Published in Advance April 1, 2011. We thank members of the Shan group for helpful comments on the manuscript. This work was supported by NIH grant GM078024 to S.S. S.S. was supported by a career award from the Burroughs Wellcome Foundation, the Beckman Young Investigator award, the Packard and Lucile award in science and engineering, and the Henry Dreyfus teacher-scholar award.

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Published - Shen2011p13641Rna.pdf

Supplemental Material - Supplementary_figure_1.doc


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