Data S1.
S
equences of the constructs used in this study
.
HA secretory sequence
Flag
-
tag
Human GPCR sequence
Universal Module
A. dLight
1.
1/dLight
1.
2
/dLight
1.
3
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILS
TLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSF
CNIWVAFDIMCSTASILNLCVISVDRYWAISSP
(
F
/A)
RYERKMTPKAAFILISVAWTLSVLI
SFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRI
YRIAQK
Q
LSSLINVYIKADKQKNGIKANFKIRHNI
EDGGVQLAYHYQQNTPIGDGPVLLP
DNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFT
GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGV
QCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
IDFKEDGNILGHKLEYNNHDQL
KRETKVLKTLSVIMGVFVCCWLPFFILNCILPF
CGSGE
TQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIETVSI
NNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPALSVIL
DYDTDVSLEKIQPITQNGQHPT
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGAGGACTCTGAACACCTCTGCCATGGACGGGACTGGGCT
GGTGGTGGAGAGGGACTTCTCTGTTCGTATCCTCACTGCCTGTTTCCTGTCGCTGCTC
ATCCTGTCCACGCTCCTGGGGAACACGCTGGTCTGTGCTGCCGTTATCAGGTTCCGA
CACCTGCGGTCCAAGGTGACCAACTTCTTTGTCATCTCCTTGGCTGTGTCAGATCTCT
TGGTGGCCGTCCTGGTCATGCCCTGGAAGGCAGTGGCTGAGA
TTGCTGGCTTCTGGC
CCTTTGGGTCCTTCTGTAACATCTGGGTGGCCTTTGACATCATGTGCTCCACTGCATC
CATCCTCAACCTCTGTGTGATCAGCGTGGACAGGTATTGGGCTATCTCCAGCCCT
TTT
CGGTATGAGAGAAAGATGACCCCCAAGGCAGCCTTCATCCTGATCAGTGTGGCATG
GACCTTGTCTGTACTCATCTCCTTCATCCCAGTGCAGCTCAGCTGGCACAAGGCAAA
ACCCACAAGCCC
CTCTGATGGAAATGCCACTTCCCTGGCTGAGACCATAGACAACTG
TGACTCCAGCCTCAGCAGGACATATGCCATCTCATCCTCTGTAATCAGCTTTTACATC
CCTGTGGCCATCATGATTGTCACCTACACCAGGATCTACAGGATTGCTCAGAAA
CAG
CTGAGCTCACTCATTAACGTCTATATCAAGGCCGACAAGCAGAAGAACGGCATCAA
GGCGAACTTCAAGATCCGCCACAACATCGAGGACGGCGGC
GTGCAGCTCGCCTACC
ACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAGCGCGATCACAT
GGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTA
CAAGGGCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGG
GTGGTGCCCATCCTGG
TCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGT
GTCCGGCGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCT
GCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACG
GCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGT
CCGCCATGCCCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAA
GGACGACGGC
AACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCAT
CGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGG
AGTACAACAATCATGACCAACTG
AAAAGAGAAACTAAAGTCCTGAAGACTCTGTCG
GTGATCATGGGTGTGTTTGTGTGCTGTTGGCTACCTTTCTTCATCTTGAACTGCATTT
TGCCCTTCTGTGGGTCTGGG
GAGACGCAGCCCTTCTGCATTGATTCCAACACCTTTG
ACGTGTTTGTGTGGTTTGGGTGGGCTAATTCATCCTTGAACCCCATCATTTATGCCTT
TAATGCTGATTTTCGGAAGGCATTTTCAACCCTCTTAGGATGCTACAGACTTTGCCCT
GCGACGAATAATGCCATAGAGACGGTGAGTATCAATAACAATGGGGCCGCGATGTT
TTCCAGCCATCATGAGCCACGAGGCTCCATCTCCAAGGAGTGCAATC
TGGTTTACCT
GATCCCACATGCTGTGGGCTCCTCTGAGGACCTGAAAAAGGAGGAGGCAGCTGGCA
TCGCCAGACCCTTGGAGAAGCTGTCCCCAGCCCTATCGGTCATATTGGACTATGACA
CTGACGTCTCTCTGGAGAAGATCCAA
CCCATCACACAAAACGGTCAGCACCCAACC
*
Mutation site: phenylalanine (F) in dLight
1.
1, Alanine (A) in dLight
1.
2
Residu
e only present in dLight
1.
3
B.
dLight
1.
4
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MGNRSTADADGLLAGRGPAAGASAGASAGLAGQG
AAALVGGVLLIGAVLAGNSLVCVSVATERALQTPTNSFIVSLAAADLLLALLVLPLFVYS
EVQGGAWLLSPRLCDALMAMDVMLCTASIFNLCAISVDRFVAVAVPLRYNRQGGSRR
QLLLIGATWLLSAAVAAPVLCGLNDVRGRDPAVCRLEDRDYVVYSSVCSFFLPCPLMLL
LYWATFRGLQR
LSSLINVYIKADKQKNGIKANFKIRHNIEDGGVQL
AYHYQQNTPIGDG
PVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKG
EELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTL
TYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRI
ELKGIDFKEDGNILGHKLEYNNHDQL
GRERKAMRVLPVVVGAFLLCWTPFFVVHITQA
LCPACSVP
PRLVSAVTWLGYVNSALNPVIYTVFNAEFRNVFRKALRACC
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGGGGAACAGATCCACTGCAGATGCAGACGGTCTTCTCGC
AGGCCGGGGACCTGCTGCCGGAGCGAGCGCTGGGGCTTCCGCAGGTCTTGCTGGGC
AGGGGGCCGCGGCCTTGGTTGGAGGCGTTTT
GCTTATAGGGGCCGTTCTTGCTGGCA
ATAGTTTGGTATGTGTTTCAGTTGCGACAGAGCGCGCACTTCAGACGCCGACTAACT
CCTTTATAGTGAGTTTGGCTGCTGCAGATCTCTTGTTGGCATTGTTGGTACTCCCACT
GTTCGTTTATTCAGAAGTACAGGGTGGCGCATGGCTCCTGTCACCCAGGTTGTGTGA
TGCCTTGATGGCCATGGATGTTATGCTGTGTACCGCTTCTATCTTTAACCTTTGTGCT
ATCAGTGTTGACAGATTCGTCGCGGTCGCGGTCCCTCTGAGGTATAACCGGCAAGGA
GGCAGCAGGAGGCAACTGCTGCTGATCGGCGCAACTTGGCTCCTCTCCGCAGCAGT
GGCCGCGCCTGTTCTGTGTGGTCTCAACGACGTTCGCGGCAGAGACCCGGCTGTATG
TCGCCTCGAGGATAGAGATTATGTCGTATACTCAAGTGTGTGTTCCTTTTTTCTTCCT
TGCCCACTGATGCTTCTGTTGTATTGGG
CTACCTTTAGAGGACTGCAACGC
CTGAGC
TCACTCATTAACGTCTATATCAAGGCCGACAAGCAGAAGAACGGCATCAAGGCGAA
CTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCTACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTG
AGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAGCGCGATCACATGGTCCT
GCT
GGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGG
GCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTG
CCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGG
CGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCA
CCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTG
ACCACCCTGACCTACGGCGTGC
AGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCA
TGCCCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTAC
AAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCT
GAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACA
ACAATCATG
ACCAACTG
GGCCGCGAACGGAAAGCCATGCGAGTTTTGCCGGTGGTA
GTAGGGGCATTCCTTCTTTGTTGGACCCCTTTTTTTGTGGTGCATATAACGCAGGCTC
TGTGCCCGGCCTGTTCTGTCCCACCCCGCCTCGTGTCAGCTGTCACTTGGTTGGGTTA
CGTAAACTCAGCCCTCAATCCAGTTATCTATACGGTTTTCAATGCCGAGTTCAGGAA
TGTTTTTAGGAAGGCC
CTTAGAGCCTGTTGT
*
C.
B1AR
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MGAGVLVLGASEPGNLSSAAPLPDGAATAARLLVP
ASPPASLLPPASESPEPLSQQWTAGMGLLMALIVLLIVAGNVLVIVAIAKTPRLQTLTNLF
IMSLASADLVMGLLVVPFGATIVVWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALD
RYLAITSPFRYQSLLTRARARGLVCTVWAISALVSFLPILMHWWRAESDEARR
CYNDPK
CCDFVTNRAYAIASSVVSFYVPLCIMAFVYLRVFREAQK
LSSLINVYIKADKQKNGIKAN
FKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEF
VTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGD
ATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQE
RTIFFKDDGNYKTR
AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHDQL
LREQK
ALKTLGIIMGVFTLCWLPFFLANVVKAFHRELVPDRLFVFFNWLGYANSAFNPIIYCRSP
DFRKAFQGLLCCARRAARRRHATHGDRPRASGCLARPGPPPSPGAASDDDDDDVVGAT
PPARLLEPWAGCNGGAAADSDSSLDEPCRPGFASESKV
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGG
TGTTCGCC
GACTACAAG
GACGATGATGACGCC
GGCGCTGGAGTCCTCGTACTGGGGGCAAGCGAACCGGGGAA
TTTGAGCTCCGCAGCACCCCTCCCGGATGGTGCGGCAACTGCTGCTCGCCTCTTGGT
CCCAGCTAGTCCGCCAGCAAGTTTGCTCCCGCCAGCGTCTGAGAGCCCCGAGCCCCT
CTCCCAGCAGTGGACTGCAGGGATGGGCTTGCTGATGGCACTCATTGTACTGTTGAT
CGTTGCGGGGAA
CGTACTTGTGATAGTGGCAATAGCTAAGACTCCCAGGCTGCAAA
CACTCACCAACCTTTTTATAATGAGTCTTGCTAGTGCTGATCTCGTAATGGGATTGCT
TGTGGTGCCTTTTGGAGCCACAATTGTAGTGTGGGGACGGTGGGAATACGGCTCCTT
TTTTTGCGAACTTTGGACTTCCGTCGATGTATTGTGCGTAACTGCCTCTATCGAGACT
CTTTGTGTCATTGCTCTTGATAGATATCTGGCCATCACT
AGCCCTTTTCGGTACCAGA
GCCTGCTGACGCGCGCAAGAGCCCGCGGGCTCGTATGTACCGTCTGGGCCATTAGTG
CTCTGGTTTCCTTTCTCCCAATTCTTATGCACTGGTGGAGAGCCGAGTCTGATGAGGC
ACGAAGGTGTTATAACGACCCTAAATGCTGCGATTTCGTGACTAATAGAGCTTATGC
TATTGCCAGCAGCGTGGTCAGTTTTTATGTCCCCCTGTGTATAATGGCCTTCGTCTAT
CTTCGGG
TATTTCGGGAAGCTCAAAAA
CTGAGCTCACTCATTAACGTCTATATCAAG
GCCGACAAGCAGAAGAACGGCATCAAGGCGAACTTCAAGATCCGCCACAACATCGA
GGACGGCGGCGTGCAGCTCGCCTACCACTACCAGCAGAACACCCCCATCGGCGACG
GCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCGTGCAGTCCAAACTTTCGAAAG
ACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGA
GTTCGTGACCGCCGCCGGG
ATCACTCTCGGCATGGACGAGCTGTACAAGGGCGGTACCGGAGGGAGCATGGTGAG
CAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCG
ACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGTGAGGGCGATGCCACCTAC
GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCC
ACCCTCGTGACC
ACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCAC
ATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACATCCAGGAGCG
CACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCG
AGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGAC
GGCAACATCCTGGGGCACAAGCTGGAGTACAACAATCATGACC
AACTG
CGAGAACA
GAAGGCCCTGAAAACACTTGGTATCATAATGGGCGTATTCACTCTTTGCTGGCTTCC
ATTTTTCCTTGCAAACGTGGTGAAAGCATTCCATCGAGAACTTGTCCCCGATAGACT
GTTCGTATTCTTCAATTGGCTGGGGTACGCTAATAGTGCCTTTAACCCTATCATCTAC
TGTCGCAGCCCCGACTTTCGAAAGGCCTTTCAAGGACTGCTGTGCTGTGCGAGGCGC
GCTGCGCGACGGAG
ACACGCCACTCATGGAGATCGCCCTCGAGCTAGCGGATGCTT
GGCCCGGCCAGGGCCCCCACCTAGTCCAGGTGCGGCGTCCGATGACGACGATGACG
ACGTGGTAGGCGCTACACCACCTGCTCGGCTCTTGGAACCGTGGGCTGGATGCAATG
GCGGTGCAGCGGCTGACTCAGATTCTTCTTTGGACGAACCATG
CAGACCCGGTTTTG
CCTCTGAGTCAAAGGTT
*
D.
B2AR
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVV
GMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHI
LMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPAKYQSLLTKNKARVII
LMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVI
MVFVYSRVFQEAKR
LSSL
INVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPI
GDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSM
VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPT
LVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDT
LVNRIELKGIDFKEDGNILGHKLEYNNHDQL
KEHKALKTL
GIIMGTFTLCWLPFFIVNIVH
VIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSRYPNVRPNNGYI
YNAHSWQSENREQSKGSSGDSDHAEGNLAKEECLSADKTDSNGNCSKAQMRVL
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGGGGCAGCCAGGTAATGGCTCTGCGTTCTTGT
TGGCCCCG
AACAGGAGCCATGCTCCCGACCATGACGTCACCCAACAGAGAGATGAGGTCTGGGT
AGTAGGCATGGGTATTGTCATGTCTCTGATAGTCTTGGCAATCGTGTTTGGAAATGT
GCTCGTTATCACGGCAATAGCTAAGTTTGAGCGACTTCAAACGGTAACAAATTATTT
CATAACATCTCTCGCGTGTGCAGATCTCGTAATGGGACTCGCTGTGGTCCCCTTTGG
CGCGGCCCATATCCTGATGAA
GATGTGGACTTTTGGTAATTTCTGGTGTGAATTTTG
GACCAGCATAGATGTACTCTGTGTTACAGCTTCAATTGAAACTCTCTGTGTGATAGC
CGTTGATCGCTATTTCGCCATTACGTCCCCTGCCAAGTATCAATCATTGCTTACCAAG
AATAAAGCCCGAGTAATAATTCTCATGGTGTGGATCGTAAGCGGGCTCACATCTTTT
TTGCCGATTCAGATGCACTGGTATAGAGCAACGCACCAAGAAGCCATA
AACTGCTA
CGCAAATGAAACTTGCTGTGACTTCTTTACAAATCAGGCTTACGCTATTGCCTCTTCA
ATAGTCAGTTTTTACGTTCCTCTGGTTATTATGGTGTTTGTATACTCACGGGTATTCC
AGGAGGCTAAGCGG
CTGAGCTCACTCATTAACGTCTATATCAAGGCCGACAAGCAG
AAGAACGGCATCAAGGCGAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGT
GCAGCTCGCCTACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCT
GCCCGACAACCACTACCTGAGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGA
AGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGGGCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGA
GCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAA
CGGCC
ACAAGTTCAGCGTGTCCGGCGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACC
CTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACC
ACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCA
CGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACATCCAGGAGCGCACCATCTTCTT
CAAGGACGACGGCAACTACAAGACC
CGCGCCGAGGTGAAGTTCGAGGGCGACACC
CTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCT
GGGGCACAAGCTGGAGTACAACAATCATGACCAACTG
AAGGAGCACAAAGCGCTG
AAGACGCTTGGAATTATCATGGGGACGTTTACTCTCTGCTGGCTTCCTTTCTTCATAG
TAAACATTGTTCACGTAATCCAAGACAATCTGATTCGAAAGGAGGTGTATATTCTCC
TCAATTGGATTGGGTACGTAAACAGCGGATTTAATCCTCTTATCTATTGCCGAAGCC
CTGATTTCCGCATAGCCTTTCAGGAACTGCTTTGTCTTCGCCGATCCCGGTATCCGAA
TGTGCGTC
CTAACAATGGCTACATTTACAATGCTCATAGCTGGCAGTCCGAAAACAG
GGAACAGAGCAAAGGTTCCAGTGGAGACAGTGACCACGCTGAAGGCAATCTCGCTA
AGGAAGAGTGCCTCAGCGCCGATAAAACTGATTCAAATGGGAATTGCAGCAAAGCT
CAAATGCGTGTTCTG
*
E.
DRD2
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MDPLNLSWYDDDLERQNWSRPFNGSDGKA
DRPHY
NYYATLLTLLIAVIVFGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVV
YLEVVGEWKFSRIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRR
VTVMISIVWVLSFTISCPLLFGLNNADQNECIIANPAFVVYSSIVSFYVPFIVTLLVYIKIYI
VLRRRRK
LSSLINVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLP
DNHYLSVQSKL
SKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFT
GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGV
QCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
IDFKEDGNILGHKLEYNNHDQL
KEKKATQMLAIVLGVFIICWLPFFITHILNIHCDCNIPPV
LYSAFTWLGYVNSAVNPIIYTTFNIEFRK
AFLKILHC
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGGACCCCCTTAACCTCTCATGGTACGACGATGATCTTGAG
AGGCAGAACTGGTCCCGACCATTCAATGGGTCTGATGGTAAGGCTGACCGGCCTCAT
TACAATTATTATGCGACCCTGCTTACTCTTCTTATCGCTGTGATCGTATTCGGCAACG
TCTTGGTTTGCATGGCAGTCTCTAGGGAAAAAGCGCTCCAGACGACAACTAATTACT
TGATTGTGAGTCTGGCTGTAGCTGACTTGCTTGTGGCGACCC
TGGTGATGCCATGGG
TCGTATACTTGGAAGTCGTTGGCGAGTGGAAGTTTTCTAGGATTCATTGCGACATAT
TTGTAACTCTGGACGTAATGATGTGTACTGCTTCCATTTTGAACCTCTGCGCTATATC
CATTGACAGGTACACGGCGGTTGCTATGCCGATGCTTTATAATACCCGGTATTCAAG
CAAAAGGCGAGTAACTGTGATGATAAGCATTGTATGGGTGCTCAGTTTCACAATTAG
CTGCCCTCTGCT
CTTCGGCCTTAACAACGCGGATCAAAATGAATGCATCATCGCAAA
CCCGGCTTTTGTGGTTTATAGCAGCATTGTTAGCTTCTATGTGCCATTCATAGTTACG
CTCCTTGTTTATATAAAAATTTATATCGTGCTTAGGCGCCGCCGAAAA
CTGAGCTCA
CTCATTAACGTCTATATCAAGGCCGACAAGCAGAAGAACGGCATCAAGGCGAACTT
CAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTC
GCCTACCACTACCAGC
AGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGC
GTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCT
GGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGGCG
GTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCC
ATCCTGGTCGAGCTGG
ACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGA
GGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCG
GCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGT
GCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGC
CCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAAGGACGACGG
CAACTACAAG
ACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAA
GGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACA
ATCATGACCAACTG
AAGGAAAAGAAGGCCACGCAAATGTTGGCAATCGTGCTCGGC
GTGTTCATAATCTGCTGGCTTCCATTTTTTATAACGCATATATTGAACATACACTGTG
ATTGCAATATTCCACCAGTC
CTGTATAGTGCGTTTACGTGGTTGGGTTATGTGAATTC
TGCGGTTAACCCGATCATTTACACCACGTTCAACATAGAATTCCGAAAGGCATTCCT
CAAAATATTGCATTGT
*
F.
A2AR
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MGSLQPDAGNASWNGTEAPGGGARATPYSLQVTL
TLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANE
V
MGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAAEYNLKRTPRRIKAIIIT
VWVISAVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYVRI
YQIAKR
LSSLINVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPD
NHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTG
VVPILVELDGDVN
GHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQ
CFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
DFKEDGNILGHKLEYNNHDQL
REKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPR
TLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGGGCTCCCTCCAGCCGGACGCAGGCAATGCGTCATGGAA
TGGGACAGAAGCCCCTGGCGGAGGCGCAAGGGCAACGCCATATAGTCTTCAAGTAA
CCCTGACTCTTGTCTGCCTCGCAGGTCTCCTGATGCTCCTGACGGTCTTTGGCAATGT
GCTTGTGATAATAGCTGTATTTACATCCA
GGGCACTCAAGGCGCCACAAAACTTGTT
TTTGGTTTCACTGGCGTCCGCCGACATTCTTGTGGCTACGCTCGTCATCCCCTTCTCA
CTCGCTAATGAAGTGATGGGTTATTGGTACTTTGGCAAGGCGTGGTGTGAGATATAT
CTTGCACTCGATGTCTTGTTCTGCACGAGCAGTATAGTTCATCTCTGCGCTATCTCAT
TGGATAGATATTGGTCAATAACGCAAGCTgcTGAGTATAACCTGAAGAGAACCCC
TA
GACGGATCAAGGCCATTATTATTACCGTTTGGGTCATATCAGCGGTCATCTCATTCC
CTCCCTTGATAAGCATTGAGAAAAAAGGTGGAGGCGGAGGACCGCAACCTGCTGAG
CCCAGATGTGAGATAAACGACCAAAAATGGTACGTTATATCAAGTTGTATCGGGTC
ATTCTTTGCGCCATGCTTGATTATGATTCTGGTATATGTTAGAATCTATCAGATAGCG
AAACGG
CTGAGCTCACTCATTAACGTC
TATATCAAGGCCGACAAGCAGAAGAACGG
CATCAAGGCGAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCG
CCTACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGAC
AACCACTACCTGAGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGA
GC
TGTACAAGGGCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTTCA
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTC
AGCGTGTCCGGCGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTT
CATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGAC
CTACGGCGTGCAGTGCTTCAGCCGCTACCCCGA
CCACATGAAGCAGCACGACTTCTT
CAAGTCCGCCATGCCCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAAGGACG
ACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAAC
CGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAA
GCTGGAGTACAACAATCATGACCAACTG
AGGGAGAAACGATTTACGTTCGTTCTCGC
CGTAGTC
ATCGGTGTTTTCGTAGTTTGCTGGTTCCCTTTCTTTTTTACATACACATTGA
CTGCCGTCGGCTGTTCTGTACCCCGAACACTTTTTAAATTTTTTTTCTGGTTCGGGTA
CTGCAACTCTAGCCTTAATCCGGTGATATACACAATTTTTAATCATGATTTTCGGCGG
GCTTTTAAGAAAATTCTCTGTCGGGGGGACCGAAAACGCATAGTG
*
G.
KOR
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MDSPIQIFRGEPGPTCAPSACLPPNSSAWFPGWAEP
DSNGSAGSEDAQLEPAHISPAIPVIITAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIY
IFNLALADALVTTTMPFQSTVYLMNSWPFGDVLCKIVISIDYYNMFTSIFTLTMMSVDRY
IAVCHPVKALDFRTPLKAKIINICIWLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDDY
SWWDLFMKICVFIFAFVIPVLIIIVCYTL
MILRLKS
LSSLINVYIKADKQKNGIKANFKIRH
NIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAA
GITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
KLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFF
KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHD
QL
REKDRNLRRI
TRLVLVVVAVFVVCWTPIHIFILVEALGSTSHSTAALAAYYFCIALGYTNAALNPILYAFL
DENFKRCFRDFCFPLKMRMERQATARVRNTVQDPAYLRDIDGMNKPV
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGGATTCACCAATTCAGATATTTAGGGGTGAACCGGGCCCT
ACCTGCGCGCCAAGTGCTTGTCTTCCTCCGAATTCAAGCGCATGGTTCCCCGGCTGG
GCAGAGCCCGACTCTAATGGGTCTGCTGGCAGTGAAGACGCGCAGCTTGAACCTGC
CCATATTAGCCCTGCTATACCCGTTATCATCACCGCCGTATATTCCGTTGTATTCGTG
GTCGGTCTGGTAGGAAATTCTCTGGTGATGTTTGTTATCATAC
GATATACCAAGATG
AAAACAGCTACCAACATCTACATCTTCAATCTGGCCTTGGCGGATGCGCTGGTCACT
ACTACTATGCCATTTCAATCAACTGTGTACCTTATGAACTCCTGGCCTTTTGGCGATG
TATTGTGCAAGATCGTCATCTCAATAGATTACTATAATATGTTTACTTCCATATTTAC
TCTCACTATGATGAGCGTCGATCGATATATAGCAGTATGTCACCCAGTAAAGGCTTT
GGACTTTCGAAC
GCCATTGAAGGCGAAAATTATAAACATTTGCATTTGGCTGCTTTC
CTCCAGCGTTGGAATCTCCGCTATTGTGTTGGGGGGGACCAAGGTCAGAGAAGACG
TAGACGTGATCGAGTGTTCCCTGCAGTTCCCGGACGATGATTATAGCTGGTGGGATC
TCTTTATGAAGATCTGTGTCTTCATCTTTGCTTTCGTAATACCTGTCCTTATCATTATT
GTATGCTATACGCTGATGATACTTAGATTGAAATCC
CTG
AGCTCACTCATTAACGTC
TATATCAAGGCCGACAAGCAGAAGAACGGCATCAAGGCGAACTTCAAGATCCGCCA
CAACATCGAGGACGGCGGCGTGCAGCTCGCCTACCACTACCAGCAGAACACCCCCA
TCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCGTGCAGTCCAAA
CTTTCGAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGAC
CGCCGCCGGGATCA
CTCTCGGCATGGACGAGCTGTACAAGGGCGGTACCGGAGGGA
GCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAG
CTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGTGAGGGCGA
TGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGT
GCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTG
CTTCAGCCGCTA
CCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACAT
CCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG
TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTC
AAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACAATCATGACCAACT
G
AGAGAGAAGGACCGGAAT
CTGCGACGCATTACCAGACTGGTCCTTGTGGTCGTGG
CAGTGTTCGTGGTCTGCTGGACTCCCATTCATATCTTTATACTGGTGGAGGCACTCGG
TTCTACGAGCCATAGCACTGCGGCACTTGCAGCTTACTACTTCTGTATTGCACTTGGG
TACACGAATGCTGCACTGAATCCTATCCTGTACGCCTTCCTTGATGAGAATTTCAAA
AGATGTTTCCGCGATTTCTGTTTCCCGTTGAAGATGCGGATGGAAA
GACAAGCTACG
GCCCGCGTTAGAAATACTGTTCAAGACCCTGCATATCTTCGGGACATAGATGGTATG
AATAAACCCGTT
*
H.
MOR
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MDSSAAPTNASNCTDALAYSSCSPAPSPGSWVNLS
HLDGNLSDPCGPNRTDLGGRDSLCPPTGSPSMITAITIMALYSIVCVVGLFGNFLVMYVI
VRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFT
SIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIINVCNWILSSAIGLPVMFMATTKYRQG
SIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGL
MILRLKS
LSSLINVYIKADKQ
KNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRD
HMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSV
SGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSA
MPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHD
QL
KEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALVTIPETTFQTVSWHFCIALGYT
NSCLNPVLYAFLDENFKRCFREFCIPTSSNIEQQNSTRIRQNTRDHPSTANTVDRTNHQLE
NLEAETAPLP
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGGATAGTAGCGCTGCGCCTACCAACGCGTCAAACT
GCAC
CGATGCTCTTGCGTACTCCTCCTGCTCCCCGGCACCTAGTCCCGGTTCTTGGGTCAAT
TTGTCCCATCTGGACGGAAACCTCTCTGATCCCTGTGGGCCTAACAGGACGGACCTC
GGTGGGAGGGACTCCCTTTGCCCGCCGACCGGATCTCCGTCCATGATAACGGCCATT
ACAATTATGGCGTTGTATAGCATCGTATGCGTTGTAGGTCTTTTTGGGAATTTCCTGG
TGATGTACGTCATCGTCAGGTA
CACAAAGATGAAAACAGCTACTAACATTTATATAT
TTAACCTGGCGCTCGCGGACGCTCTCGCAACGTCAACGCTCCCGTTTCAGTCCGTGA
ATTATCTCATGGGTACTTGGCCTTTCGGAACAATACTGTGTAAAATTGTTATAAGCA
TAGATTATTATAATATGTTCACGTCCATCTTCACACTCTGcACAATGTCTGTGGATAG
GTACATTGCTGTATGTCACCCAGTTAAGGCGCTTGACTTTAGAACTCCA
CGCAATGC
AAAGATTATAAATGTGTGCAACTGGATCTTGTCCTCTGCAATAGGGCTTCCTGTGAT
GTTCATGGCGACTACTAAGTACAGACAGGGCAGCATAGATTGcACACTCACCTTCTC
ACACCCAACTTGGTACTGGGAAAATCTGCTCAAGATCTGCGTCTTCATTTTTGCTTTT
ATCATGCCAGTATTGATAATCACGGTCTGTTACGGGTTGATGATTTTGCGGCTCAAA
TCA
CTGAGCTCACTCATTA
ACGTCTATATCAAGGCCGACAAGCAGAAGAACGGCAT
CAAGGCGAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCT
ACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAAC
CACTACCTGAGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAGCGCGATCA
CATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGA
CGAGCT
GTACAAGGGCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTTCACC
GGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAG
CGTGTCCGGCGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCA
TCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCT
ACGGCGTGCAGTGCTTCAGCCGCTAC
CCCGACCACATGAAGCAGCACGACTTCTTCA
AGTCCGCCATGCCCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAAGGACGAC
GGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCG
CATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGC
TGGAGTACAACAATCATGACCAACTG
AAGGAGAAGGACCGCAACCTCAGAAGGATA
A
CGAGAATGGTGCTGGTCGTAGTGGCGGTTTTCATTGTTTGTTGGACGCCAATACAC
ATATACGTGATTATAAAGGCTCTGGTGACAATTCCCGAAACAACGTTTCAGACGGTC
TCTTGGCATTTCTGTATTGCATTGGGGTACACTAATTCCTGCCTTAATCCTGTATTGT
ACGCCTTTCTGGATGAAAACTTTAAAAGATGTTTCCGCGAGTTCTGCATACCGACCA
GCAGCAACATTGAACAACAAAACTCCAC
GCGCATACGGCAAAATACTAGGGATCAC
CCGTCCACTGCGAATACTGTAGACCGAACGAACCATCAGTTGGAGAATTTGGAAGC
GGAAACTGCTCCTCTGCCA
*
I.
5HT2A
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGE
ANTSDAFNWTVDSENRTNLSCEGCLSPSCLSLLHLQEKNWSALLTAVVIILTIAGNILVIM
AVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRWPLPSKLCAVWIYLD
VLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQD
DSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFL
TIKSLQK
LSSLINVYIKADKQKN
GIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHM
VLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGE
GEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPE
GYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHD
QL
N
EQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNEDVIGALLNVFVWIGYLSSAVNP
LVYTLFNKTYRSAFSRYIQCQYKENKKPLQLILVNTIPALAYKSSQLQMGQKKNSKQDA
KTTDNDCSMVALGKQHSEEASKDNSDGVNEKVSCV
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGGACATACTT
TGTGAAGAGAATACTTCACTCTCTTCTACT
ACTAACTCTCTTATGCAACTGAACGATGATACCCGATTGTACTCAAACGACTTCAAT
TCCGGCGAAGCGAACACCAGTGACGCATTCAACTGGACTGTCGATTCTGAAAACAG
AACTAATCTGTCATGCGAGGGTTGTCTTAGTCCCTCTTGTCTCAGCCTGTTGCACCTC
CAGGAAAAGAACTGGTCAGCACTGCTCACTGCGGTAGTGATAATACTCACTATTG
CT
GGCAATATTCTCGTAATTATGGCAGTCTCCTTGGAGAAGAAACTCCAAAACGCCACA
AATTATTTTCTTATGAGCCTTGCCATCGCAGATATGCTCTTGGGATTTTTGGTGATGC
CTGTGAGTATGCTCACGATACTGTATGGATATCGCTGGCCTCTGCCGTCTAAaCTTTG
CGCTGTGTGGATTTACTTGGATGTCCTTTTTTCAACTGCGAGTATTATGCATCTTTGC
GCCATTAGTCTTGATAGGTATGT
GGCTATCCAAAATCCTATACACCATTCCCGCTTTA
ATAGTAGAACTAAGGCTTTTCTGAAAATAATAGCAGTGTGGACCATATCTGTCGGCA
TAAGCATGCCTATCCCCGTATTTGGACTTCAAGATGACTCAAAGGTATTCAAAGAAG
GGTCATGTCTGCTGGCCGATGACAATTTCGTGCTTATTGGATCCTTCGTCAGTTTCTT
CATTCCTTTGACAATCATGGTGATTACCTACTTTCTTACGATTAAATCT
TTGCAAAAG
CTGAGCTCACTCATTAACGTCTATATCAAGGCCGACAAGCAGAAGAACGGCATCAA
GGCGAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCTACC
ACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAGCGCGATCACAT
GGTCCTGCTGGAGTTCGTGACCG
CCGCCGGGATCACTCTCGGCATGGACGAGCTGTA
CAAGGGCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGG
GTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGT
GTCCGGCGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCT
GCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CG
GCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGT
CCGCCATGCCCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAAGGACGACGGC
AACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCAT
CGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGG
AGTACAACAATCATGACCAACTG
AATGAG
CAAAAGGCTTGTAAGGTACTCGGCATA
GTCTTCTTTCTGTTTGTGGTGATGTGGTGTCCCTTCTTTATAACGAATATCATGGCAG
TGATCTGCAAGGAATCATGCAATGAGGATGTGATCGGGGCACTTCTGAACGTTTTCG
TGTGGATAGGGTATCTGTCAAGTGCTGTGAACCCACTGGTCTATACCTTGTTTAATA
AGACATACCGcTCAGCCTTTTCACGGTATATTCAATGTCAGTATAAGGAAAACAAGA
AACCTCTGCAACTTATTCTTGTGAACACTATCCCTGCCCTGGCTTATAAGTCATCACA
GTTGCAGATGGGCCAGAAAAAAAATTCCAAGCAGGACGCGAAGACAACAGACAAC
GATTGTAGTATGGTTGCCCTCGGCAAGCAGCACAGTGAAGAAGCGAGCAAAGACAA
TAGTGATGGCGTAAACGAAAAAGTCAGTTGTGTA
*
J.
MT2
Aminoacid sequence
MKTIIALSYIFCLVFA
DYKDDDDA
MSENGSFANCCEAGGWAVRPGWSGAGSARPSRTP
RPPWVAPALSAVLIVTTAVDVVGNLLVILSVLRNRKLRNAGNLFLVSLALADLVVAFYP
YPLILVAIFYDGWALGEEHCKASAFVMGLSVIGSVFNITAIAINRYCYICHSMAYHRIYR
RWHTPLHICLIWLLTVVALLPNFFVGSLEYDPRIYSCTFIQTASTQYTAAVVVIHFLLPIA
VVSFCYLRIWVLVLQARR
LSSLINVYIKADKQKNGIKANFKI
RHNIEDGGVQLAYHYQQ
NTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTG
GSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVP
WPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKF
EGDTLVNRIELKGIDFKEDGNILGHKLEYNNHDQL
KPSDLRSFLTMFVVFVIFAICWAPL
NCIG
LAVAINPQEMAPQIPEGLFVTSYLLAYFNSCLNAIVYGLLNQNFRREYKRILLALW
NPRHCIQDASKGSHAEGLQSPAPPIIGVQHQADAL
*
DNA sequence
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCC
GACTACAAG
GACGATGATGACGCC
ATGTCAGAAAACGGATCTTTCGCGAATTGTTGCGAAGCAGG
GGGATGGGCCGTGAGGCCAGGCTGGTCTGGAGCAGGTTCTGCCAGGCCCTCAAGGA
CGCCGAGACCTCCTTGGGTAGCTCCTGCTCTGTCCGCTGTGCTGATAGTGACCACTG
CTGTGGACGTCGTAGGCAATCTTCTCGTGATCTTGTCTGTCCTGCGAAATCGGAAAC
TTAGGAACGCCGGAAATTTGTTCCTGGTCTCATTGGCGCTCGCAG
ACCTGGTAGTTG
CCTTCTATCCGTACCCCCTCATTTTGGTGGCTATCTTTTACGACGGCTGGGCCCTGGG
TGAGGAGCATTGCAAAGCATCTGCCTTCGTAATGGGTCTGAGCGTGATTGGCTCTGT
ATTTAATATCACGGCCATAGCCATCAACAGGTACTGCTACATATGCCACAGTATGGC
TTACCATCGGATCTACCGCAGATGGCACACACCTCTTCACATATGCCTGATATGGCT
CTTGACGGTCGTCGC
ATTGCTCCCAAATTTCTTCGTAGGATCATTGGAGTACGACCC
AAGGATCTACTCCTGCACGTTTATTCAGACTGCGTCAACCCAATATACCGCAGCCGT
GGTGGTAATACACTTCCTCCTGCCTATAGCCGTCGTTAGCTTTTGCTATCTGAGGATT
TGGGTTCTTGTACTCCAGGCCAGGCGA
CTGAGCTCACTCATTAACGTCTATATCAAG
GCCGACAAGCAGAAGAACGGCATCAAGGCGAACTTCAAGATC
CGCCACAACATCGA
GGACGGCGGCGTGCAGCTCGCCTACCACTACCAGCAGAACACCCCCATCGGCGACG
GCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCGTGCAGTCCAAACTTTCGAAAG
ACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGG
ATCACTCTCGGCATGGACGAGCTGTACAAGGGCGGTACCGGAGGGAGCATGGTGAG
CAAGGGCGAGGAGCTGT
TCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCG
ACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGTGAGGGCGATGCCACCTAC
GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCC
ACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCAC
ATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACAT
CCAGGAGCG
CACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCG
AGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGAC
GGCAACATCCTGGGGCACAAGCTGGAGTACAACAATCATGACCAACTG
AAGCCTTC
CGATCTTCGGAGCTTTCTTACAATGTTTGTAGTCTTCGTCATCTTTGCTATATGCTGG
GCCCCGTTGAATTGTATAGGA
CTTGCGGTGGCCATCAACCCTCAAGAAATGGCTCCC
CAGATTCCAGAAGGCCTGTTTGTCACCAGTTACCTCCTCGCATACTTCAACTCCTGCC
TTAACGCCATAGTCTACGGGTTGCTGAACCAAAACTTCCGACGCGAGTACAAGAGA
ATTTTGCTGGCTCTCTGGAATCCGCGCCATTGCATCCAGGATGCGTCTAAAGGCTCA
CACGCAGAAGGACTCCAGTCTCCAGCGCCTCCAATTATAGGCGTCCAAC
ATCAAGC
GGATGCGCTT
*