Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published October 29, 2018 | Published
Journal Article Open

Synthetic Bessel light needle for extended depth-of-field microscopy


An ultra-long light needle is highly desired in optical microscopy for its ability to improve the lateral resolution over a large depth of field (DOF). However, its use in image acquisition usually relies on mechanical raster scanning, which compromises between imaging speed and stability and thereby restricts imaging performance. Here, we propose a synthetic Bessel light needle (SBLN) that can be generated and scanned digitally by complex field modulation using a digital micromirror device. In particular, the SBLN achieves a 45-fold improvement in DOF over its counterpart Gaussian focus. Further, we apply the SBLN to perform motionless two-dimensional and three-dimensional microscopic imaging, achieving both improved resolution and extended DOF. Our work is expected to open up opportunities for potential biomedical applications.

Additional Information

© 2018 Published by AIP Publishing. Received 17 September 2018; accepted 20 October 2018; published online 2 November 2018. The authors acknowledge financial support from National Institutes of Health (NIH) Grant Nos. DP1 EB016986 (NIH Director's Pioneer Award), R01 NS102213 (PEDBRAIN), U01 NS090579 (BRAIN1 Initiative), and U01 NS099717 (BRAIN2 Initiative).

Attached Files

Published - 1.5058163.pdf


Files (1.5 MB)
Name Size Download all
1.5 MB Preview Download

Additional details

September 15, 2023
October 23, 2023