Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published January 10, 2014 | Published + Accepted Version + Supplemental Material
Journal Article Open

Photoimprint Photoacoustic Microscopy for Three-Dimensional Label-Free Subdiffraction Imaging


Subdiffraction optical microscopy allows the imaging of cellular and subcellular structures with a resolution finer than the diffraction limit. Here, combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for subdiffraction photoacoustic imaging of both fluorescent and nonfluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. The differential signal between the two excitations preserves the signal contribution mostly from the center of the excitation volume, and dramatically sharpens the lateral resolution. Moreover, due to the nonlinear nature of the signal, our method offers an inherent optical sectioning capability, which is lacking in conventional photoacoustic microscopy. By scanning the excitation beam, we performed three-dimensional subdiffraction imaging of varied fluorescent and nonfluorescent species. As any molecules have absorption, this technique has the potential to enable label-free subdiffraction imaging, and can be transferred to other optical imaging modalities or combined with other subdiffraction methods.

Additional Information

© 2014 American Physical Society. Received 19 September 2013; published 10 January 2014. The authors appreciate support by Professor James Ballard and useful discussions with Konstantin Maslov, Amos Danielli, and Arie Krumholz. This research was supported by the National Institutes of Health Grants No. DP1 EB016986, No. R01 EB008085, No. R01 CA134539, No. U54 CA136398, No. R01 CA157277, and No. R01 CA159959.

Attached Files

Published - PhysRevLett.112.014302.pdf

Accepted Version - nihms551264.pdf

Supplemental Material - PI_PAM_PRL_supp_revision2_v1.docx

Supplemental Material - YaoMovieS1.avi

Supplemental Material - YaoMovieS2.avi

Supplemental Material - YaoMovieS3.avi

Supplemental Material - YaoMovieS4.avi


Files (10.2 MB)
Name Size Download all
1.2 MB Preview Download
1.5 MB Download
304.4 kB Download
284.2 kB Download
392.1 kB Download
1.9 MB Preview Download
4.6 MB Download

Additional details

August 19, 2023
October 19, 2023