An Electrochemical Probe of DNA Stacking in an Antisense Oligonucleotide Containing a C3'-endo-Locked Sugar
The preferred base-stacking orientation of a conformationally constrained nucleotide (3′-endo-locked) within DNA/DNA and DNA/RNA duplexes (see picture) was probed by charge transport through DNA-modified gold electrode surfaces. The conformation of the sugar is seen to sensitively determine the local stacking of the duplex. These results illustrate the utility of DNA-mediated charge transport through DNA-modified surfaces in characterizing small perturbations in DNA stacking and structure.
© 2002 WILEY-VCH Verlag GmbH & Co. Received: March 14, 2002. Article first published online: 16 Sep 2002. This work was supported bythe National Institutes of Health (GM61077 to J.K.B.), the Swedish Natural Science Research Council (N.F.R.), the Swedish Research Council for Engineering Sciences (T.F.R.), and the Stiftelsen för Strategisk forskning (J.C.).