Supporting Information
Effects of the Photooxidant on DNA-Mediated Charge Transport
Tashica T. Williams, Chikara Dohno, Eric D. A. Stemp,
and Jacqueline K. Barton
*
Division of Chemistry and Chemical Engineering
California Institute of Technology, Pasadena, CA 91125
Supporting Figure 1
. HPLC profiles of dG and d
CP
G oxidation by photoexcited
thionine. From top to bottom: a degassed solution of dG (250
μ
M) in 10 mM sodium
phosphate (pH 7.6) and 50 mM NaCl, irradiated for 4 minutes at 599 nm in the presence
of thionine (25
μ
M) (top). A degassed solution of d
CP
G (250
μ
M) in 10 mM sodium
phosphate (pH 7.6) and 50 mM NaCl, irradiated for 4 minutes at 599 nm in the presence
of thionine (25
μ
M) (middle). An aerated solution of d
CP
G (250
μ
M) in 10 mM sodium
phosphate (pH 7.6) and 50 mM NaCl, irradiated for 4 minutes at 599 nm in the presence
of thionine (25
μ
M) (bottom).
Supporting Figure 2
. HPLC profiles of nucleoside mixtures obtained from enzymatic
digestion of irradiated
CP
G-containing DNA assemblies functionalized with different
photooxidants. In all cases, results are shown for the assembly shown in Figure 1
derivatized with
CP
G at the distal site. (A) The
CP
G-DNA/AQ 2 duplex (5
μ
M ) in 10
mM sodium phosphate (pH 7) was irradiated for one minute at 350 nm followed by
enzymatic digestion. (B) The
CP
G/Ru assembly (5
μ
M) in 20 mM Tris-Cl (pH 8.1), 10
mM NaCl, and 25
μ
M Co(NH
3
)
5
Cl
2+
was irradiated for ten minutes at 436 nm followed
by enzymatic digestion. (C) The
CP
G/Rh assembly (5
μ
M) in 20 mM Tris-Cl (pH 8.1), 10
mM NaCl was irradiated at 365 nm for ten minutes followed by enzymatic digestion.
Supplemental Figure 1
Supplemental Figure 2