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Published July 8, 2003 | Published
Journal Article Open

The human Bloom syndrome gene suppresses the DNA replication and repair defects of yeast dna2 mutants


Bloom syndrome is a disorder of profound and early cancer predisposition in which cells become hypermutable, exhibit high frequency of sister chromatid exchanges, and show increased micronuclei. BLM, the gene mutated in Bloom syndrome, has been cloned previously, and the BLM protein is a member of the RecQ family of DNA helicases. Many lines of evidence suggest that BLM is involved either directly in DNA replication or in surveillance during DNA replication, but its specific roles remain unknown. Here we show that hBLM can suppress both the temperature-sensitive growth defect and the DNA damage sensitivity of the yeast DNA replication mutant dna2-1. The dna2-1 mutant is defective in a helicase-nuclease that is required either to coordinate with the crucial Saccharomyces cerevisiae (sc) FEN1 nuclease in Okazaki fragment maturation or to compensate for scFEN1 when its activity is impaired. We show that human BLM interacts with both scDna2 and scFEN1 by using coimmunoprecipitation from yeast extracts, suggesting that human BLM participates in the same steps of DNA replication or repair as scFEN1 and scDna2.

Additional Information

© 2003 by the National Academy of Sciences. Edited by Charles C. Richardson, Harvard Medical School, Boston, MA, and approved May 6, 2003 (received for review March 20, 2003). This paper was submitted directly (Track II) to the PNAS office. We thank Dr. Norma Neff for hBLM gene alleles and anti-hBLM antibody and for critical reading of the manuscript; Alexander Varshavsky for critical reading of the manuscript; Dr. Y. Furuichi for hBLM, mWRN, and hRECQ4 genes; and N. Nobuo for the hDNA2 EST clone. This work was supported by U.S. Public Health Service Grant GM25508.

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