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Published December 1, 1973 | public
Journal Article Open

The Presence of Ribonucleotides in Mature Closed-Circular Mitochondrial DNA


Mitochondrial DNA from mouse and HeLa cells is nicked by alkali and chick-embryo ribonuclease H. It has been concluded that ribonucleotides are present in the closed-circular duplex. A comparative study of the scission rates at pH 13.0 of RNA and of mitochondrial DNAs indicates that there are no more than 10 ribonucleotides in the major fraction of the mitochondrial DNAs.

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Copyright © 1973 by the National Academy of Sciences Contributed by Jerome Vinograd, June 7, 1973 We thank Dr. W. Keller for samples of RNase H and Dr. J. Hurwitz for a preprint of his manuscript. We also thank J. Edens and D. Stephens for assistance with cell preparations. This investigation was supported in part by USPHS Grant CA08014 from the National Cancer Institute and Grant GM15327 from the National Institute of General Medical Sciences. L.I.G. was a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. This is contribution no. 4706 from the Division of Chemistry and Chemical Engineering.


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