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Published January 14, 2014 | Accepted Version
Journal Article Open

Fabrication of cell patches using biodegradable scaffolds with a hexagonal array of interconnected pores (SHAIPs)


Cell patches are widely used for healing injuries on the surfaces or interfaces of tissues such as those of epidermis and myocardium. Here we report a novel type of porous scaffolds made of poly(d,l-lactic-co-glycolic acid) for fabricating cell patches. The scaffolds have a single layer of spherical pores arranged in a unique hexagonal pattern and are therefore referred to as "scaffolds with a hexagonal array of interconnected pores (SHAIPs)". SHAIPs contain both uniform pores and interconnecting windows that can facilitate the exchange of biomacromolecules, ensure homogeneous cell seeding, and promote cell migration. As a proof-of-concept demonstration, we have created skeletal muscle patches with a thickness of approximately 150 μm using SHAIPs. The myoblasts seeded in the scaffolds maintained high viability and were able to differentiate into multi-nucleated myotubes. Moreover, neovasculature could efficiently develop into the patches upon subcutaneous implantation in vivo.

Additional Information

© 2013 Elsevier Ltd. Received 17 April 2013; Received in revised form 10 June 2013; Accepted 11 June 2013; Available online 20 June 2013. This work was supported by an NIH Director's Pioneer Award (DP1 OD000798) and startup funds from Georgia Institute of Technology (to Y.X.). This work was also sponsored by NIH grants (R01 EB000712, R01 NS46214, R01 EB008085, and U54 CA136398, to L.V.W.). Part of the research was performed at the Alafi Neuroimaging Laboratory, the Hope Center for Neurological Disorders, which is supported by the NIH Neuroscience Blueprint Center Core Grant P30 NS057105. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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Accepted Version - nihms497178.pdf


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