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Published April 1, 2015 | public
Journal Article Open

Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells


We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite–phosphoprotein correlation network.

Additional Information

© 2015 American Chemical Society. Received: January 28, 2015. Publication Date (Web): March 19, 2015. This work was supported by the National Cancer Institute Grant No. 5U54 CA151819 (JRH PI), the Ben and Catherine Ivy Foundation, the Jean Perkins Foundation, and the Phelps Family Foundation. We acknowledge Professor Michael Phelps for helpful discussions regarding glucose assays. Author Contributions: M.X. and W.W. contributed equally. The authors declare no competing financial interest.

Attached Files

Supplemental Material - ja5b00944_si_001.pdf

Accepted Version - nihms787682.pdf


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August 20, 2023
August 20, 2023