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Published October 15, 2018 | Accepted Version
Journal Article Open

Dual-view photoacoustic microscopy for quantitative cell nuclear imaging


Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions—determined optically and acoustically, respectively—are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.

Additional Information

© 2018 Optical Society of America. Received 25 July 2018; accepted 31 August 2018; posted 10 September 2018 (Doc. ID 340705); published 3 October 2018. D. Cai appreciates the support from China Scholarship Council. L. V. Wang has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. Funding: National Institutes of Health (NIH) (DP1 EB016986, R01 CA186567, U01 NS090579, U01 NS099717).

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August 19, 2023
October 19, 2023