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Published June 1977 | Published
Journal Article Open

The quantitation of fluorescence by photography


A method based on theory has been developed for the photographic quantitation of fluorescent substances. DNA stained with ethidium in agarose gels is used as an example of an application of this method. In the course of developing this method we have demonstrated that the empirical methods employed by other authors can give rise to large systematic errors. We have also developed an approximate method based on photographic theory, avoiding the use of digital integration which is required by the rigorous method.

Additional Information

© 1977 Oxford University Press. Received January 1, 1977. We wish to thank Dr. M. Ross and M. Klymkowsky for their help in calibrating the step density wedges. This work was supported in part by USPHS, National Institutes of Health Grants GM15327 and CA 08014. D. E. P. is a recipient of a Medical Research Council of Canada fellowship. M. S. is a recipient of an N. S. F. graduate fellowship. This is contribution No. 5426 from the Division of Chemistry and Chemical Engineering.

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