CaltechAUTHORS
Published December 1, 2002 | Version Published
Journal Article Open

Targeted Derepression of the Human Immunodeficiency Virus Type 1 Long Terminal Repeat by Pyrrole-Imidazole Polyamides

Creators

Abstract

The host factor LSF represses the human immunodeficiency virus type 1 long terminal repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-imidazole polyamides targeted to the LTR can specifically block LSF binding both in vitro and within cells via direct access to chromatin, resulting in increased LTR expression.

Additional Information

© 2002 American Society for Microbiology. Received 4 June 2002/ Accepted 28 August 2002 We thank H. Johnson for excellent technical assistance. We thank R. Koup and R. Gaynor for advice and J. V. Garcia, D. Sodora, and U. Hansen for advice and careful review. The following reagents were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH): HeLaCD4-CAT from Barbara K. Felber and George N. Pavlakis and ACH-2 from Thomas Folks. This work was supported by an NIH postdoctoral grant (GM19789-02) to C.M., an NIH grant (AI 45297) to D.M.M., and an NIH grant (GM51747) to P.B.D.

Attached Files

Published - COUjvir02.pdf

Files

COUjvir02.pdf

Files (1.5 MB)

Name Size Download all
md5:0dd1cb0db171bfb631d778f3cf96f451
1.5 MB Preview Download

Additional details

Identifiers

PMCID
PMC136904
Eprint ID
2231
Resolver ID
CaltechAUTHORS:COUjvir02

Funding

NIH Postdoctoral Fellowship
GM19789-02
NIH
AI-45297
NIH
GM-51747

Dates

Created
2006-03-17
Created from EPrint's datestamp field
Updated
2023-06-01
Created from EPrint's last_modified field