In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging
The purpose of this study is to map non-invasively sentinel lymph nodes (SLNs) and lymphatic vessels of rats in vivo using FDA-approved indocyanine green (ICG) and two non-ionizing imaging modalities: volumetric spectroscopic photoacoustic (PA) imaging, which measures optical absorption, and planar fluorescence imaging, which measures fluorescent emission. SLNs and lymphatic vessels were clearly visible after a 0.2 ml-intradermal-injection of 1 mM ICG in both imaging systems. We also imaged deeply positioned lymph nodes in vivo by layering biological tissues on top of rats. These two modalities, when used together with ICG, have the potential to map SLNs in axillary staging and to study tumor metastasis in breast cancer patients.
Additional Information© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was supported in part by grants from National Institutes of Health (R01 EB000712, R01 EB008085, R01 NS46214, and U54 CA136398 -- the Network for Translational Research -- to L.V.W.). L.V.W. has a financial interest in Endra, Inc., which, however, did not support this work.
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