© 202
1
Wiley
-
VCH GmbH
Supporting Information
for
Adv. Sci.
,
DOI: 10.1002/ad
vs
.
202102474
Multiscale
photoacoustic tomography of
a
genetically encoded
near
-
infrared FRET biosensor
Lei Li
,
Hsun
-
C
hia Hsu
,
Vladislav V. Verkhusha
,
Lihong V. Wang
*
,
and
Daria M.
Shcherbakova
*
Multiscale photoacoustic tomography of
a
genetically encoded near
-
infrared FRET biosensor
Lei Li,
Hsun
-
C
hia Hsu, Vladislav V. Verkhusha, Lihong V. Wang
,
and Daria M. Shcherbakova
Supplementary Information
Supplementary
Figure 1.
The background is generated by heme
-
binding proteins in cultured
cells.
Normalized
absorbance
spectra of heme
-
binding proteins
(hemoglobin and cytochrome b)
and
normalized photoacoustic signals
of HeLa cells were overlayed. The photoacoustic signal is
directly proportional to the
absorption
of molecule
s
.
Several cytochrome proteins are
found in
mammalian cells: cytochromes a, a3, b, and c
45
.
W
e present here the spectra of the most
abundant cyto
chrome b (43% of cyt
ochrome
b, 21% cyt
ochrome
c, and 36% of more red
-
shifted
cyt
ochrome
a
,a3
). The signal
s
from
non
-
transfected
HeLa cells
in vitro
were imaged at different
wavelengths using the PACT
(
setup described in the Main text
)
. Mean values and SEM
were
normalized and plotted.