Letter
https://doi.org/10.1038/s41586-019-1053-2
Chemosensory modulation of neural circuits for
sodium appetite
Sangjun Lee
1
, Vineet Augustine
1
, Yuan Zhao
1
, Haruka ebisu
1
, Brittany Ho
1
, Dong Kong
2
& Yuki Oka
1
*
1
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
2
Department of Neuroscience, Tufts University School of Medicine, Boston, MA, USA.
*
e-mail:
yoka@caltech.edu
NATURE|
www.nature.com/nature
SUPPLEMENTARY INFORMATION
https://doi.org/10.1038/s41586-019-1053-2
In the format provided by the authors and unedited.
Figure Number
Sample size
(n = mice
otherwise
stated)
Test
P value
1a; Control vs - Sodium
5,5
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0015
1e; Empty vs NaCl
4,10
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0204
1e; Water vs NaCl
10,10
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0002
1f Total bout duration (s); - Sodium vs - Light
4,8
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0038
1f Total bout duration (s); - Light vs + Light
8,8
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0031
1f # of bout; - Sodium vs - Light
4,8
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0018
1f # of bout; - Light vs + Light
8,8
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0046
1g; Non-stim vs co-stim
8,8
Friedman test (Dunn's multiple
comparisons test)
0.0009
1h; - Light vs + Light
7,7
two-tailed Wilcoxon test
0.0156
2a, Session 1; eYFP vs ChR2
8,10
two-way repeated measure ANOVA
(Sidak's multiple comparisons test)
0.0016
2a, Session 2; eYFP vs ChR2
8,10
two-way repeated measure ANOVA
(Sidak's multiple comparisons test)
0.0117
2a, Session 3; eYFP vs ChR2
8,10
two-way repeated measure ANOVA
(Sidak's multiple comparisons test)
<0.0001
2b, ChR2; +light vs -light
6,6
two-tailed Wilcoxon test
0.0313
3c left; water vs 0.06M NaCl
8,7
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0041
3c left; water vs 0.15M NaCl
8,7
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0088
3c left; water vs 0.5 M NaCl
8,7
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0016
3d; KCl vs NaCl
9,9
two-tailed Wilcoxon test
0.0039
3e; + Ami vs - Ami
9,9
two-tailed Wilcoxon test
0.0039
4b left; Control vs NaCl Oral
7,7
Friedman test (Dunn's multiple
comparisons test)
0.0323
4b middle; Control vs Water IG
8,8
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0221
4b middle; Control vs Water oral
8,6
Kruskal-Wallis test (Dunn's multiple
comparisons test)
0.0003
4b right; Control vs Glucose IG
8,8
two-tailed Wilcoxon test
0.0078
4e left; NaCl IG vs NaCl Oral
7,7
Friedman test (Dunn's multiple
comparisons test)
0.0113
4e left; Water IG vs NaCl Oral
7,7
Friedman test (Dunn's multiple
comparisons test)
0.0214
4e left; Air IG vs NaCl Oral
7,7
Friedman test (Dunn's multiple
comparisons test)
0.0057
5b left; + Casp3 vs - Casp3
18, 8
two-tailed Mann-Whitney test
<0.0001
5b right; + Casp3 vs - Casp3
18, 8
two-tailed Mann-Whitney test
<0.0001
5f; + Ami vs - Ami
7,7
two-tailed Wilcoxon test
0.0469
Supplementary Information - Table 1: Statistics
Extended Data 1b -Water; Water vs 0.5M NaCl
9,9
two-tailed Wilcoxon test
0.0039
Extended Data 1b -Sodium; Water vs 0.5M NaCl
9,9
two-tailed Wilcoxon test
0.0039
Extended Data 2a left, 0.06 M NaCl; eYFP vs ChR2
5,4
two-tailed Mann-Whitney test
0.0159
Extended Data 2a right, 0.15 M NaCl; eYFP vs ChR2
5,4
two-tailed Mann-Whitney test
0.0159
Extended Data 2c left, KCl; NaCl vs KCl
9,9
Friedman test (Dunn's multiple
comparisons test)
0.0044
Extended Data 2c left, KCl; KCl vs NaCl
9,9
Friedman test (Dunn's multiple
comparisons test)
0.0019
Extended Data 2c right, Ami; - Ami vs + Ami
8,8
Friedman test (Dunn's multiple
comparisons test)
0.0009
Extended Data 2c right, Ami; - Ami vs + Ami
8,8
Friedman test (Dunn's multiple
comparisons test)
0.0248
Extended Data 3g left, - Sodium; Vehicle vs CNO
9,9
two-tailed Wilcoxon test
0.0078
Extended Data 4c; - Light vs + Light of Session 4
6,6
two-tailed Wilcoxon test
0.0313
Extended Data 5d right, NaCl; eYFP vs GCaMP6s
7,7
two-tailed Mann-Whitney test
0.007
Extended Data 5d right, Empty; eYFP vs GCaMP6s
4,4
two-tailed Mann-Whitney test
0.0286
Extended Data 5e left, KCl; KCl vs NaCl
9,9
two-tailed Wilcoxon test
0.0039
Extended Data 5e left, Ami; + Ami vs - Ami
9,9
two-tailed Wilcoxon test
0.0273
Extended Data 6b; - Light vs + Light
6,6
two-tailed Mann-Whitney test
0.0065