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Published March 2012 | public
Book Section - Chapter

A free-standing and flexible parylene PCR device


We present a free-standing and flexible parylene-channel PCR device that allows a simple sealing mechanism to prevent bubbling caused by PCR temperature cycling. This sealing mechanism saves complicated, time-consuming in-channel valve fabrication processes and achieves excellent sealing performance. It is proven experimentally that this new PCR device plus its unique packaging is capable of locking PCR solution in the PCR chamber at 95°C for at least 5 hours without bubbling and leaking. As a result, we have successfully obtained the on chip PCR amplification without the bubble and leaking problem.

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© 2012 IEEE. This work was supported by The Royal Thai Government scholarship and The Boeing Company. The authors appreciate all generous help and precious advice from the members of Caltech Micromachining Laboratory.

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August 22, 2023
October 23, 2023