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Published April 25, 2005 | Published
Book Section - Chapter Open

High-resolution photoacoustic vascular imaging in vivo using a large-aperture acoustic lens


Reflection-mode photoacoustic microscopy with dark-field laser pulse illumination and high frequency ultrasonic detection is used to non-invasively image blood vessels in the skin in vivo. Dark-field illumination minimizes the interference caused by strong photoacoustic signals from superficial structures. A high numerical-aperture acoustic lens provides high lateral resolution, 45-120 micrometers in this system while a broadband ultrasonic detection system provides high axial resolution, estimated to be ~15-20 micrometers. The optical illumination and ultrasonic detection are in a coaxial confocal configuration for optimal image quality. The system is capable of imaging optical-absorption contrast at up to 3 mm depth in biological tissue.

Additional Information

© 2005 Society of Photo-Optical Instrumentation Engineers (SPIE). This project was sponsored by the National Institutes of Health grants R01 EB000712 and R01 NS46214.

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