Visualization of RNA tertiary struture by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNAPhe with uridine-EDTA·Fe(II) at position 47
To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA.Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)(2)(SO4)(2) and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.
© 1994 by the National Academy of Sciences. Contributed by Peter B. Dervan, February 24, 1993. We are grateful to the National Institutes of Health for grant support. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Published - HANpnas94.pdf