SUPPLEMENTAL MATERIAL
Supplemental
Fig
ure
S
1
.
Oxygen consumption rate (OCR) of the spheroids.
OCRs of
primary pancreatic
islets and pseudo
-
islets (PsIs) derived from the insulin
-
secreting cell line
were measured under the well
-
oxygenated condition.
Primary pancreatic islets were collected from five individual rats (n = 5).
OCRs of PsIs
were measured from the individual preparations (n = 7). The data was normalized by the volume of the spheroids
applied for the measurements.
The data were
presented as mean ± standard error of the mean (SEM)
.
Supplemental
Fig
ure
S2.
Live/dead images in various size of spheroids and
distribution of the spheroid
size analyzed in the study
.
A
: Representative live/dead images of primary islets in various sizes, approximately
radius at 50, 100 and 150 μm. Pre
-
culture (top) and post
-
culture (bottom). Scale bar: 100 μm.
B
: Representative
live/dead images of pseudo
-
islets (PsIs) in various sizes, approximately radius at 50, 100 and 150 μm. Pre
-
culture (top) and post
-
culture (bottom). Scale bar: 100 μm.
C
: Size distribution of the primary islets analyzed in
this study, categorized by the radius in 10 μm pitch. D: Size distribution of the PsIs analyze
d in this study,
categorized by the radius in 10 μm pitch.
E
: Scatter plots of individual primary islets in their viability versus size
(n = 262).
F
: Scatter plots of individual PsIs in their viability versus size (n = 107).
Supplemental
Fig
ure
S
3
.
The critical survival pO
2
within spheroids
.
Scatter plots of individual spheroids
with the information of
volume of spheroid (
v
spheroid
)
and critical survival pO
2
in primary islets (left panel
,
n = 262
spheroids) and in PsIs (right panel, n = 107
spheroids)
.
Primary islets
Pseudo-islets (PsIs)