Localization of actin in Dictyostelium amebas by immunofluorescence
Antibody prepared against avian smooth muscle actin has been used to localize actin in the slime mold, Dictyostelium discoideum. The distribution of actin in migrating cells is different from that in feeding cells. Migrating amebas display fluorescence primarily in advancing regions whereas feeding amebas show uniform fluorescence throughout. The reaction is specific for actin since the fluorescence observed is blocked when the antibody is absorbed by actin purified from avian skeletal muscle, human platelets, and Dictyostelium. These results, in addition to describing the distribution of actin in D. discoideum, demonstrate that actins from these diverse sources share at least one common antigenic determinant.
Additional Information© 1978 by The Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 9 December 1976, and in revised form 31 October 1977. The authors wish to express their gratitude to Dr. Keith R. Porter, in whose laboratory this work was done. Dr. Eckert was supported by grant RR 00592 from the National Institutes of Health to Dr. Porter. Dr. Lazarides was supported by a post-doctoral fellowship from the Muscular Dystrophy Association and by grant GM 06965 from the National Institutes of Health.
Published - ECKjcb78.pdf