CNTF, FGF, and NGF collaborate to drive the terminal differentiation of MAH cells into postmitotic neurons
The differentiation of neuronal cell progenitors depends on complex interactions between intrinsic cellular programs and environmental cues. Such interactions have recently been explored using an immortalized sympathoadrenal progenitor cell line, MAH. These studies have revealed that depolarizing conditions, in combination with exposure to FGF, can induce responsiveness to NGF. Here we report that CNTF, which utilizes an intracellular signaling pathway distinct from that of both FGF and NGF, can collaborate with FGF to promote efficiently the differentiation of MAH progenitor cells to a stage remarkably reminiscent of NGF-dependent, postmitotic sympathetic neurons. We also find that similar collaborative interactions can occur during transdifferentiation of normal cultured chromaffin cells into sympathetic neurons.
© 1994 by Cell Press. Received 19 October 1993, Revised 20 May 1994, Available online 22 April 2004. We thank Drs. L. S. Schleifer and the entire Discovery group at Regeneron for their enthusiastic support and insightful scientific input and Karen Mattsson, Li Pan, and Joyce McClain in particular for their expert technical support. D. J. A. is an Associate Investigator and S. J. B. is an Associate of the Howard Hughes Medical Institute. J. M. V. is supported by an NRSA from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC Section 1734 solely to indicate this fact.