Single-cell photoacoustic thermometry
A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With 3 seconds per frame imaging speed, a temperature resolution of 0.2 °C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.
Additional Information© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored by the National Institutes of Health (NIH) under grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, R01 CA159959 and DP1 EB016986. L. V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc.; however, neither provided support for this work.
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