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Published April 12, 2023 | Published + Supplemental Material
Journal Article Open

Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate nervous system


Modern neuroscience approaches including optogenetics, calcium imaging, and other genetic manipulations have facilitated our ability to dissect specific circuits in rodent models to study their role in neurological disease. These approaches regularly use viral vectors to deliver genetic cargo (e.g., opsins) to specific tissues and genetically-engineered rodents to achieve cell-type specificity. However, the translatability of these rodent models, cross-species validation of identified targets, and translational efficacy of potential therapeutics in larger animal models like nonhuman primates remains difficult due to the lack of efficient primate viral vectors. A refined understanding of the nonhuman primate nervous system promises to deliver insights that can guide the development of treatments for neurological and neurodegenerative diseases. Here, we outline recent advances in the development of adeno-associated viral vectors for optimized use in nonhuman primates. These tools promise to help open new avenues for study in translational neuroscience and further our understanding of the primate brain.

Additional Information

© 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Research in the Gradinaru and Fox labs is funded in part by Aligning Science Across Parkinson's [ASAP-020495] through the Michael J. Fox Foundation for Parkinson's Research (MJFF) (to V.G. and A.S.F.). For the purpose of open access, the author has applied a CC BY public copyright license to all Author Accepted Manuscripts arising from this submission. Additional support was provided by the California National Primate Research Center, NIH BRAIN Initiative Armamentarium grant UF1MH128336 (to V.G. and A.S.F.) and NIH P51OD011107 (to A.S.F). Figures were created using images from BioRender.com. We would like to thank Catherine Oikonomou and Rothem Kovner for help with manuscript editing. Data availability: No data was used for the research described in the article. CRediT authorship contribution statement: Lillian J. Campos: Writing – original draft, Writing – review & editing. Cynthia M. Arokiaraj: Writing – original draft, Writing – review & editing. Miguel R. Chuapoco: Writing – review & editing. Xinhong Chen: Writing – review & editing. Nick Goeden: Writing – review & editing. Viviana Gradinaru: Writing – original draft, Writing – review & editing. Andrew S. Fox: Writing – original draft, Writing – review & editing. Declaration of competing interest: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Viviana Gradinaru reports a relationship with Capsida Biotherapeutics that includes: board membership. Nick Goeden reports a relationship with Capsida Biotherapeutics that includes: employment.

Attached Files

Published - 1-s2.0-S2665945X23000141-main.pdf

Supplemental Material - 1-s2.0-S2665945X23000141-mmc1.pdf


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Additional details

August 22, 2023
December 22, 2023