Diversity of the G-protein family: sequences from five additional α subunits in the mouse
Biochemical analysis has revealed a number of guanine nucleotide-binding regulatory proteins (G proteins) that mediate signal transduction in mammalian systems. Characterization of their cDNAs uncovered a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of diversity of the G protein family, we used the polymerase chain reaction to detect additional gene products in mouse brain and spermatid RNA that share these conserved regions. Sequences corresponding to six of the eight known G protein alpha subunits were obtained. In addition, we found sequences corresponding to five newly discovered alpha subunits. Our results suggest that the complexity of the G protein family is much greater than previously suspected.
Additional Information© 1989 National Academy of Sciences. Contributed by Melvin I. Simon, July 11, 1989. We thank Ryn Miake-Lye, Gregg Gundersen, and Anthony Bellvd for RNA; Narasimhan Gautam for valuable discussions, George Komatsoulis for computer analysis; and Mike Lochrie and Andy Pakula for critical reading of the manuscript. This work was supported by the National Science Foundation and the National Institutes of Health.
Published - STRApnas89.pdf