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Published January 2017 | public
Book Section - Chapter

Gas perfusable microfabricated membranes for high-density cell culture


We report for the first time the design, fabrication, and feasibility of a high-density cell culture system capable of a cell substrate density of 40 cm^2/cm^3, greater than 250× current commercially available systems. This design provides oxygen directly from gas perfusable membranes on which the cells are grown to overcome oxygen delivery limitations in traditional culture systems. With translation in mind, the membranes are composed only of medical grade PDMS and Parylene to mitigate contaminant leaching concerns.

Additional Information

© 2017 IEEE. Thanks to the Caltech MEMS Lab members for their guidance with techniques and fabrication. In memory of Lillian Boasie and Gweneth Goodman.

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August 19, 2023
October 24, 2023