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Published July 1, 1985 | public
Journal Article Open

Effect of ARS1 mutations on chromosome stability in Saccharomyces cerevisiae


We have used a set of deletion mutations in the ARS1 element of Saccharomyces cerevisiae to measure their effect on chromosome stability. This work establishes the previously proposed existence of three domains in ARS1. Domain C, which we have previously inferred, but not proved, to be a part of ARS1, is now established. In addition, we show that increasingly large deletions of the domain have increasingly large effects, which was not realized before. Furthermore, we have provided the first positive evidence for the central importance of a 14-base-pair core sequence containing the ARS consensus element by showing that it has the ability to act as a replicator on a plasmid containing no other ARS1 flanking sequence. The method of analyzing plasmid stability used in our study employs a novel and sensitive flow cytometry assay for beta-galactosidase. We discuss ways in which flow cytometry, based on this assay, could be generalized beyond its particular application in this work to studying other aspects of the cell biology of yeast and higher cells. The actual flow cytometry method will be described in detail elsewhere.

Additional Information

Copyright © 1985 by the American Society for Microbiology. Received 17 December 1984/Accepted 8 April 1985 We thank Dan Stinchcomb, Mark Rose, and Leonard Guarente for plasmids, Stewart Scherer and Mark Johnston for yeast strains, and Doug Koshland for comments on the manuscript. This investigation was supported by Public Health Service grant GM25588 from the National Institutes of Health and by grants from the American Cancer Society, the March of Dimes, the National Science Foundation, and the ECUT program of the Department of Energy.


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