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Published October 15, 2010 | public
Journal Article

Portable Filter-Based Microdevice for Detection and Characterization of Circulating Tumor Cells


Purpose: Sensitive detection and characterization of circulating tumor cells (CTC) could revolutionize the approach to patients with early-stage and metastatic cancer. The current methodologies have significant limitations, including limited capture efficiency and ability to characterize captured cells. Here, we report the development of a novel parylene membrane filter-based portable microdevice for size-based isolation with high recovery rate and direct on-chip characterization of captured CTC from human peripheral blood. Experimental Design: We evaluated the sensitivity and efficiency of CTC capture in a model system using blood samples from healthy donors spiked with tumor cell lines. Fifty-nine model system samples were tested to determine the recovery rate of the microdevice. Moreover, 10 model system samples and 57 blood samples from cancer patients were subjected to both membrane microfilter device and CellSearch platform enumeration for direct comparison. Results: Using the model system, the microdevice achieved >90% recovery with probability of 95% recovering at least one cell when five are seeded in 7.5 mL of blood. CTCs were identified in 51 of 57 patients using the microdevice, compared with only 26 patients with the CellSearch method. When CTCs were detected by both methods, greater numbers were recovered by the microfilter device in all but five patients. Conclusions: This filter-based microdevice is both a capture and analysis platform, capable of multiplexed imaging and genetic analysis. The microdevice presented here has the potential to enable routine CTC analysis in the clinical setting for the effective management of cancer patients.

Additional Information

© 2010 American Association for Cancer Research. Received April 29, 2010. Revision received July 8, 2010. Accepted July 12, 2010. H.K. Lin and S. Zheng contributed equally. H.K. Lin, S. Zheng, and A.J. Williams performed the experiments. All authors contributed to experimental design, data analysis, and manuscript writing. We thank the participating patients for the source of clinical blood samples. Grant Support: National Institutes of Health Grant 1R21CA123027 (RJC), Doheny Eye Institute Specialized Imaging Core grant EY03040. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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August 19, 2023
October 20, 2023