De Novo Design of Bioactive Protein-Resembling Nanospheres via Dendrimer-Templated Peptide Amphiphile Assembly
Self-assembling peptide amphiphiles (PAs) have been extensively used in the development of novel biomaterials. Because of their propensity to form cylindrical micelles, their use is limited in applications where small spherical micelles are desired. Here we present a platform method for controlling the self-assembly of biofunctional PAs into spherical 50 nm particles using dendrimers as shape-directing scaffolds. This templating approach results in biocompatible, stable protein-like assemblies displaying peptides with native secondary structure and biofunctionality.
Additional Information© 2011 American Chemical Society. Received: June 30, 2011. Revised: July 27, 2011. Published: August 01, 2011. This work was supported by the MRSEC Program of the NSF under Award No. DMR05-20415, the National Institutes of Health as a Program of Excellence in Nanotechnology under Award Nos. NIH1U01 HL080718, HHSN268201000046C, the CCNE Program of the National Institutes of Health under Award No. NIH1U54 CA119335, and the UC Berkeley College of Engineering. M.J.R thanks the DOE Office of Science for a Graduate Fellowship. The authors thank P. Allen for the artistic renderings, the R. Segalman lab for use of their DLS, and the T. Xu lab for use of their AFM. The authors declare no competing financial interests.
Accepted Version - nihms-315671.pdf
Supplemental Material - nl202220q_si_001.pdf