1
Supplementary Information for
Silicate biomineralization of sediment
-
and carbonate
-
hosted anaerobic methane
-
oxidizing consortia and implications for microbial fossil preservation
Daniela Osorio
-
Rodriguez, Kyle S. Metcalfe, Shawn Erin McGlynn, Hang Yu, Anne E. Dekas, Mark
Ellisman, Tom Deerinck,
Ludmilla Aristilde,
John P. Grotzinger, and Victoria J. Orphan
This PDF file includes:
Figures S1 to S
7
Legends for Datasets S1
to S4
Other supplementary materials for this manuscript include the following:
Datasets S1
to
S
4
2
Figure
S
1
|
Correlative epifluorescence microscopy and EDS analysis of ANME
-
SRB consortia
clusters from sediment
-
free AOM enrichment cultures.
A
-
D)
DAPI
-
stained consortia clusters at
different magnifications showing the gross morphology of the clusters.
(E
-
H
)
Corresponding
SEM
-
EDS data documenting the spatial proximity of Si
-
rich phases (blue) with carbon
-
rich AOM
consortia (yellow).
I
-
J)
SEM images of silica rich spherules at 2 magnifications.
I)
Enlargement
3
of the red box area in B and F, where a
region enriched in silica nanoparticles is highlighted with
dashed red oval.
J)
Enlargement of the blue box area in D and H, illustrating Si
-
rich silicates
surrounding an ANME
-
SRB consortium.
4
5
Figure
S2 |
EDS compositional data showing the difference in aluminum, silica, and iron in
Si
-
rich
phases attached to ANME
-
SRB consortia recovered directly from methane seep sediments
(letter A) or grown under sediment
-
free conditions (letter F), compared with the composition of
the source sediment (letter S). Details on sampling locations can be found
in Methods.
Figure
S3 |
Fe
-
Al
-
Si ternary plot of EDS compositional data for
Si
-
rich phases
attached to AOM
consortia sourced directly from Santa Monica Basin methane seep sediments or from sediment
-
free AOM consortia enrichment cultures
,
plotted alongside reported compositions of silicates
attached to bacterial cell walls.
Figure
S4
|
Predicted mineral stability diagram for silicate mineral formations in the
experimental solution composition with
only
illite
and
smectite
included as clay
minerals
.
Feldspar refers to albite; Chlorite(1) and Chlorite(2) refers to chamosite and clinochlore,
respectively
; and the red bar corresponds to ICP
-
MS measurement of
[Si] in artificial seawater