of 8
Supplemen
tar
y
Information
for
"Genomic
diversity
acros
s
the Rickettsia
and 'Candidatus
Megaira'
genera
and
proposal
of genus
status
for the Torix
group
.
"
Helen R
Davison
1
, Jack
Pilgrim
1
, Nicky
Wybouw
2
, Joseph
Parker
3
, Stacy
Pirro
4
, Simon
Hunter-
Barnett
1
, Paul M
Campbell,
1,5
, Frances
Blow
1,6
, Alistair
C Darby
1
, Gregory
D D Hurst
1
and
Stefanos
Siozios
1
*
Affiliations
1.
Institut
e of In
fec
tio
n, V
eterin
ary
an
d Ecological
sc
iences
, Uni
versit
y of Liverpo
ol, L
iverp
ool, L
69
7ZB, Uni
ted King
dom
2.
Te
rrest
ria
l Ecology Uni
t, Depar
tmen
t of Bi
ology, Fac
ulty
of Scienc
es,
Gh
ent Uni
versit
y, Gh
ent,
Belg
ium
3.
Division of Bio
logy and
Biological
Enginee
rin
g, Calif
ornia In
stitute
of Te
chn
ology, 1
200 E Cali
fornia
Bo
ulevard
, Pasad
ena,
CA
91125, US
A
4.
Iridian Genome
s, Bethesda, M
D, U
SA
5.
School of Hea
lth
and Life S
ciences, Fac
ulty of Biology Me
dicine and Hea
lth,
the Un
ivers
ity of
Ma
nchester, Ma
nchester,
Uni
ted Kingdom
6.
Cent
er
for G
eno
mics
an
d Sy
ste
ms Bio
logy, D
epar
tment o
f Bio
logy, Ne
w
York
Universit
y, Ne
w
York,
New
York,
USA
*
Co
rrespo
nding auth
or (si
ozioss@l
iverp
ool.ac.u
k)
u
a:'.
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200000
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RiCimp
Supplementary
Figure
1. Whole
genome
alignment
between
the complete
Torix
limoniae
(RiCimp)
and Torix
Leech
(RiClec)
genomes
reveals
lack of synteny.
Magenta
represents
forward
matches
and blue
reverse
matches
ht
tps://doi.org/10.6084/m9.figshare.14866263.
a)
b)
135
75000
LF888_00395
3400
� 3200
3000
NRPS/PKS
hybrid cluster
3500
3250
3000
2750
2500
2250
LF885_01360
-
�----------�
NRPS gene cluster
LF888_00385
1600
1500
1400
'
e
Q
1300
LF888_00400
2050
2000
I
1950
s
t
1900
1850
1900
1800
2000
1900
Domains
@=
Ketosynthase
@
=Condensation
@
=Ketore
ductase
@
=Adenyl
ation
@
=Acyltransferase
@
=Epimerisation
Taxon
$
a-proteobacteria
$
b-proteobacteria
Q
=Thiolation
e
=N-methylation
$
CFB group
bacteria
$
Cyanobacteria
$
d-proteobacteria
Enterobacteria
$
Firmicutes
$
g-proteobacteria
$
GNS
bacteria
LF888 _ 00390
I
+
LF888_00405
$
Supplementary Figure
2
. The circular chromosomes of a) a Torix group Rickettsia (RiCimp) and b) a Ca. Megaira sp. (MegNEIS296). From
outside to in, the circles represent: forward CDSs (Red), Reverse CDSs (blue), tRNAs (yellow) rRNAs (black), and GC content (green and
magenta). Highlighted are the predicted domains that form
non-ribosomal peptide synthase (NRPS) and hybrid non-ribosomal peptide synthase/
polyketide synthase (NRPS/PKS) gene modules. Modules define individual amino acids in the synthesised peptide and show the catalytic domains
within modules. The plots beneath the predicted domains show the taxonomic distribution of the best blast hits for each predicted protein in the two
NRPS systems (LF885_01360: n=49; LF888_00385: n=86, LF888_00390: n=69; LF888_00395: n=49, LF888_00400: n=80; LF888_00405: n=71).
Center line, m
edian; box limits,
25th and 75th percentiles
; whiskers,
±
1.5x interquartile range; p
oints, data points
.
For the full resolution image:
https://doi.org/10.6084/m9.figshare.14865570.