Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published September 5, 2005 | Published
Journal Article Open

Practical reconstruction method for bioluminescence tomography


Bioluminescence tomography (BLT) is used to localize and quantify bioluminescent sources in a small living animal. By advancing bioluminescent imaging to a tomographic framework, it helps to diagnose diseases, monitor therapies and facilitate drug development. In this paper, we establish a direct linear relationship between measured surface photon density and an unknown bioluminescence source distribution by using a finite-element method based on the diffusion approximation to the photon propagation in biological tissue. We develop a novel reconstruction algorithm to recover the source distribution. This algorithm incorporates a priori knowledge to define the permissible source region in order to enhance numerical stability and efficiency. Simulations with a numerical mouse chest phantom demonstrate the feasibility of the proposed BLT algorithm and reveal its performance in terms of source location, density, and robustness against noise. Lastly, BLT experiments are performed to identify the location and power of two light sources in a physical mouse chest phantom.

Additional Information

© 2005 Optical Society of America. Received 11 July 2005; revised 16 August 2005; accepted 18 August 2005. This work is supported by an NIH/NIBIB grant EB001685, a special grant for bioluminescent imaging from Department of Radiology, College of Medicine, University of Iowa. Dr. Ming Jiang was also supported in part by NKBRSF (2003CB716101) and NSFC (60325101, 60272018 and 60372024), China.

Attached Files

Published - oe-13-18-6756.pdf


Files (1.4 MB)
Name Size Download all
1.4 MB Preview Download

Additional details

August 19, 2023
October 23, 2023