Optical-resolution photoacoustic microscopy of ischemic stroke
A major obstacle in understanding the mechanism of ischemic stroke is the lack of a tool to noninvasively or minimally invasively monitor cerebral hemodynamics longitudinally. Here, we applied optical-resolution photoacoustic microscopy (OR-PAM) to longitudinally study ischemic stroke induced brain injury in a mouse model with transient middle cerebral artery occlusion (MCAO). OR-PAM showed that, during MCAO, the average hemoglobin oxygen saturation (sO2) values of feeder arteries and draining veins within the stroke core region dropped ~10% and ~34%, respectively. After reperfusion, arterial sO_2 recovered back to the baseline; however, the venous sO_2 increased above the baseline value by ~7%. Thereafter, venous sO_2 values were close to the arterial sO_2 values, suggesting eventual brain tissue infarction.
Additional Information© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored by National Institutes of Health Grants R01 EB000712, R01 EB008085, R01 CA134539, R01 NS48283, P01 NS32636, U54 CA136398, and 5P60 DK02057933. Prof. Lihong V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
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