Biochemistry, 1998, 37(46), 16093-16105, DOI:10.1021/bi981798q
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Copyright © 1998 American Chemical Society
©
1998
American
Chemical
Society,
Biochemistry,
Franklin
bi981798q
Supporting
Info
Page
1
Supplementary
Material
Differential
DNA
Recognition
by
the
Enantiomers
of
1-Rh(MGP)2phi:
A
Combination
of
Shape
Selection
and
Direct
Readout
Sonya
J.
Franklin
and
Jacqueline
K.
Barton*
Beckman
Institute,
Division
of
Chemistry
and
Chemical
Engineering,
California
Institute
of
Technology,
Pasadena,
CA
91125
Figure
Sl:
Expanded
downfield
(imino)
region
of
Li
and
of
A-Rh:L1
90:10
H
2
0:D
2
0
NOESY
spectra,
each
collected
at
10*C
with
tmix
=
300
msec.
The
change
in
chemical
shift
of
duplex
protons
upon
binding
is
indicated
by
arrows.
Figure
S2:
Contour
plot
of
the
aromatic
region
of
a
400
msec
ROESY
spectrum
of
1:1
A-Rh:D1/D2
in
D
2
0
(1.25
mM
duplex
and
complex;
10
mM
phosphate).
The
spectrum
was
collected
at
10
'C
on
a
600
MHz
Varian
Unity
Plus
spectrometer.
Diagonal
and
exchange
peaks
are
depicted
in
black,
NOE
peaks
are
depicted
in
red.
Figure
S3:
Expanded
downfield
(imino)
region
of
Dl/D2
and
of
A-Rh:D1/D2
90:10
H
2
0:D
2
0
NOESY
spectra,
each
collected
at
10*C
with
tmix
=
300
msec.
Sets
of
exchanging
peaks
are
indicated
by
brackets.
SEP
1 0
1998
E
T
3
"i
C;.I
L1
C3NH
C3NH
G8
0
0
A6
A4
0
I
A2
S12.5
1.0
11.5
9.0
8.8.0
7.5
70
.5
0
tn
vi)
oI
.
As
6A4
0
14.0
13.5
13.0
12.5
.0
11.5
9.0
8.5
.o
7.5
7.0
6.5
ppm
0.
0.
0
'4.
QTs
T7a
A-Rh+L1
G8
T7
.
0
*t
O
O
0
0
OO
*
a
'*1
o
-
.
-
OD.
7.
**.0
5.0
-oLoa
C6NH
C13N
v
C3NH
C6NH
CN
C0
C13NH
C3NH
C
CO9NH
A91
8.5
0
Ci9NiH
A16
A4
O
A14
8.0
7.5
7.0
6.5
D1/D2
Gl8
G15
Gs
G2
T5
T17
T7
T12
0
0
LO
0
14.0
13.5
13.0
12.5
12.0
11.5
9.0
8.5
8.0
7.5
7.0
6.5
A-Rh+D1/D2
Gis
11
9
phiNH
O
phiNH
@
phi-A16
G8
C3NH
0
I
0
oC13NH[
TG2
.004
C19NH
4
o
A4
T17
0
'b9
0
0
T7
o
Al6
mTA9
T0
I
I
//
A14
LA
c~j
rjm
14.0
13.5
13.0
12.5
12.0
11.5
9.0
©
I,
00
-t
0
0
0
0
0
0
0
0
0
r:j~
-t
-t
00
I,
00
0
-t
0
0