Sample
(p
mol ala/μL
)
Procedural
Blank #
First Step
% of signal relative to
Murchison
Max
pmol
ala/μL
Σ12C
Counts
Σ13C
Counts
Σ12C
Intensity
Methods
Development
Murchison,
January
2018
(29)
Hexane
Pure Hexane
0.1%
0.0%
0.0%
0.11
1
Transfer of Meteorite to GC vial
Run Prior to Derivatization
0.5%
0.1%
0.2%
0.45
2
Extraction of meteorite at NASA
0.8%
0.2%
0.2%
2.4
3
Transfer of Meteorite to GC vial
0.3%
0.0%
0.1%
1.0
Methods
Development
Murchison,
March 2018
(29)
Hexane
Pure
Hexane
0.0%
0.1%
0.0%
3.1 x
10
-
3
1
Transfer of Meteorite to GC vial
Run Prior to Derivatization
0.2%
0.1%
0.1%
5.4 x
10
-
2
2
Extraction of meteorite at NASA
1.0%
0.0%
0.3%
0.29
3
Transfer of Meteorite to GC vial
5.6%
3.4%
3.7%
1.6
Analytical
Murchison,
Summer
2018
(15)
Hexane
Pure Hexane
0.0%
0.0%
0.0%
1.3 x
10
-
3
1
Transfer of Meteorite to GC vial
Run Prior to Derivatization
0.0%
0.0%
0.1%
1.8 x
10
-
3
2
Extraction of meteorite at NASA
1.0%
0.0%
0.2%
0.16
3
Transfer of
Meteorite to GC vial
0.7%
0.0%
0.1%
9.8 x
10
-
2
4
Chemical Derivatization
0.5%
0.0%
0.0%
6.9 x
10
-
2
Table S1.
Blank IDs and possible contamination to Murchison.
Analysis Set
Date
Scan Range
% of signal
relative to
alanine
Maximum ‰
change in
13
R
Σ
12
C
Counts
Σ
13
C
Counts
Methods
Development
Murchison,
January
2018
1/3
135
-
146
RE
0.08%
0.05%
-
0.061
1/3
135
-
146
RE
0.31%
0.29%
-
0.037
1/3
135
-
146
RE
0.16%
0.14%
-
0.027
1/4
50
-
300
DE
0.00%
0.00%
0.000
1/6
50
-
300
DE
0.04%
0.00%
-
0.091
1/7
50
-
300
DE
0.09%
0.00%
-
0.086
1/7
50
-
300
DE
0.31%
0.10%
-
0.210
1/7
181
-
187
RE
0.00%
0.05%
0.048
Methods
Development
Murchison,
March 2018
3/26
50
-
500
DE
0.0%
0.0%
-
0.002
3/26
50
-
500
DE
0.0%
0.0%
-
0.007
3/27
135
-
146
RE
0.0%
0.0%
0.000
3/29
181
-
187
RE
0.0%
0.2%
0.199
3/30
181
-
187
RE
0.0%
0.3%
0.282
3/31
181
-
187
RE
0.0%
2.8%
2.756
Analytical
Murchison,
Summer
2018
6/27
135
-
146
RE
0.12%
0.12%
-
0.002
6/27
135
-
146
RE
0.12%
0.11%
-
0.010
6/27
135
-
146
RE
0.16%
0.16%
-
0.002
7/12
181
-
187
RE
0.52%
0.49%
-
0.033
7/12
181
-
187
RE
0.00%
0.07%
0.045
7/12
181
-
187
RE
0.04%
0.01%
-
0.033
7/13
181
-
187
RE
0.05%
0.04%
-
0.013
7/16
108
-
118
RE
5.52%
6.20%
0.644
Table S2.
Background from blanks
during measurement. RE denotes samples analyzed using
Reservoir Elution mode while DE denotes samples analyzed using Direct Elution mode.
Analysis Set
Sample
Analysis Number used
140
184
Winter 2018
Alfa Aesar
28, 29
47
-
49, 51
-
53, 61
-
62
Strecker
30
64
-
67
Methods Development
Murchison
24
-
25
45
-
46, 55
-
59
Spring 2018
Alfa Aesar
75, 83
94, 97
-
99, 104
Strecker
74, 80
x
Methods Development
Murchison
78, 84
-
86
101
-
103, 105
-
106
Summer
2018
Alfa Aesar
128, 131, 134
-
137
162,
166
-
167
Strecker
139
-
140
159, 161
Analytical Murchison
142, 144, 147
164
-
165
Table S3.
Analysis numbers of measurements used to calculate
13
R values from samples and
standards. Analysis numbers correspond to values found in
Dataset S1
and were used to calculate
ratios found in Table S2
.