Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published March 1, 2005 | Published
Journal Article Open

Deeply penetrating photoacoustic tomography in biological tissues enhanced with an optical contrast agent


Photoacoustic tomography (PAT) in a circular scanning configuration was developed to image deeply embedded optical heterogeneity in biological tissues. While the optical penetration was maximized with near-infrared laser pulses of 800-nm wavelength, the optical contrast was enhanced by Indocyanine Green (ICG) dye whose absorption peak matched the laser wavelength. This optimized PAT was able to image objects embedded at depths of as much as 5.2 cm, 6.2 times the 1/e optical penetration depth, in chicken breast muscle at a resolution of <780 µm and a sensitivity of <7 pmol of ICG in blood. The resolution was found to deteriorate slowly with increasing imaging depth. The effects of detection bandwidth on the quality of images acquired simultaneously by four different ultrasonic transducers are described.

Additional Information

© 2005 Optical Society of America. Received September 15, 2004. We thank G. Stoica for assistance with blood samples and X. Xie, X. Wang, M. Sivaramakrishnan, and K. Song for laboratory assistance. This study is sponsored in part by National Institutes of Health grants R01 EB000712 and R01 NS46214 and by Texas Advanced Technology Program grant 000512-0063-2001.

Attached Files

Published - ol-30-5-507.pdf


Files (517.4 kB)
Name Size Download all
517.4 kB Preview Download

Additional details

August 19, 2023
October 23, 2023