Thermal Mechanisms of Millimeter Wave Stimulation of Excitable Cells
Interactions between millimeter waves (MMWs) and biological systems have received increasing attention due to the growing use of MMW radiation in technologies ranging from experimental medical devices to telecommunications and airport security. Studies have shown that MMW exposure alters cellular function, especially in neurons and muscles. However, the biophysical mechanisms underlying such effects are still poorly understood. Due to the high aqueous absorbance of MMW, thermal mechanisms are likely. However, nonthermal mechanisms based on resonance effects have also been postulated. We studied MMW stimulation in a simplified preparation comprising Xenopus laevis oocytes expressing proteins that underlie membrane excitability. Using electrophysiological recordings simultaneously with 60 GHz stimulation, we observed changes in the kinetics and activity levels of voltage-gated potassium and sodium channels and a sodium-potassium pump that are consistent with a thermal mechanism. Furthermore, we showed that MMW stimulation significantly increased the action potential firing rate in oocytes coexpressing voltage-gated sodium and potassium channels, as predicted by thermal terms in the Hodgkin-Huxley model of neurons. Our results suggest that MMW stimulation produces significant thermally mediated effects on excitable cells via basic thermodynamic mechanisms that must be taken into account in the study and use of MMW radiation in biological systems.
© 2013 by the Biophysical Society. Submitted March 21, 2013, and accepted for publication May 7, 2013. The authors thank John E. Carlstrom for the kind loan of the GHz signal generator, and Jorge E. Sanchez-Rodriguez for helpful discussions and reagents related to the sodium-potassium pump. This research was supported by the Miller Research Institute (to MGS), NIH grant GM030376 (to FB) and F31 NIH-NS081954 (to MFP). All four authors planned the study, designed and performed experiments, and analyzed and interpreted the data. M.G.S. and M.F.P. wrote the manuscript with input from F.B. and P.H.S.
Supplemental Material - mmc1.pdf