Published August 1969
| Version Published
Journal Article
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DNA of Bacteriophage PM2: A Closed Circular Double-Stranded Molecule
Abstract
The DNA molecules isolated from mature bacteriophage PM2 are closed double-stranded rings of a molecular weight of six million. Direct evidence for the circularity and supercoiling of PM2 DNA was obtained by electron microscopy. Other properties, such as sedimentation behavior at neutral and alkaline pH, unusually high buoyant density in alkaline CsCl, and stability upon heating, are consistent with this structure. Only the four common nucleotides are found after hydrolysis and chromatographic analysis of PM2 DNA.
Additional Information
© 1969 by the National Academy of Sciences. Communicated May 26, 1969 This research was supported in part by grant GM 13554 from the U.S. Public Health Service. M. lysodeikticus DNA and crab dAT DNA were generous gifts from Dr. J. Vinograd. We should like to acknowledge the assistance of Mr. Patrick Koen in performing the electron microscopy.Attached Files
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Additional details
Identifiers
- PMCID
- PMC223444
- Eprint ID
- 5813
- Resolver ID
- CaltechAUTHORS:ESPpnas69
Funding
- NIH
- GM 13554
Dates
- Created
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2006-11-02Created from EPrint's datestamp field
- Updated
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2023-10-16Created from EPrint's last_modified field