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Published May 17, 2017 | Supplemental Material + Accepted Version + Published
Journal Article Open

Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex


The successful planning and execution of adaptive behaviors in mammals may require long-range coordination of neural networks throughout cerebral cortex. The neuronal implementation of signals that could orchestrate cortex-wide activity remains unclear. Here, we develop and apply methods for cortex-wide Ca^(2+) imaging in mice performing decision-making behavior and identify a global cortical representation of task engagement encoded in the activity dynamics of both single cells and superficial neuropil distributed across the majority of dorsal cortex. The activity of multiple molecularly defined cell types was found to reflect this representation with type-specific dynamics. Focal optogenetic inhibition tiled across cortex revealed a crucial role for frontal cortex in triggering this cortex-wide phenomenon; local inhibition of this region blocked both the cortex-wide response to task-initiating cues and the voluntary behavior. These findings reveal cell-type-specific processes in cortex for globally representing goal-directed behavior and identify a major cortical node that gates the global broadcast of task-related information.

Additional Information

© 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Open Access funded by the US Department of Defense (DoD) or performed by an employee of DoD. Received 17 June 2016, Revised 26 January 2017, Accepted 11 April 2017, Available online 17 May 2017. We would like to thank C. Ramakrishnan for molecular biology assistance and for cloning the CAG-DIO-GCaMP6f construct, J. Fernandez-Alcudia (Stanford Gene Vector and Virus Core) for producing AAV-PHP.B, H. Zeng (Allen Institute for Brain Science) for providing transgenic mice prior to publication, J. Sanders (Cold Spring Harbor Laboratory) for providing Bpod code and hardware designs, T. Komiyama (University of California, San Diego) for providing initial olfactometer advice, N. Young for image acquisition code, Z. Guo and K. Svoboda (Janelia Research Campus) for advice on the surgical preparation, and C. Manalac for assistance with genotyping. We are grateful to members of the K.D. and L.L. laboratories for comments and advice, particularly M. Lovett-Barron, P. Rajasethupathy, C. Kim, and A. Andalman. V.G. is a Heritage Principal Investigator supported by the Heritage Medical Research Institute. AAV-PHP.B work was funded by NIH grant DP2/PECASE (V.G.) and by the Beckman Institute at Caltech (V.G. and B.E.D.) through the Resource Center on CLARITY, Optogenetics, and Vector Engineering. K.D. and L.L. are Investigators of the Howard Hughes Medical Institute and are supported by a Hughes Collaborative Innovation Award. W.E.A. and I.V.K. were supported by National Science Foundation Graduate Research Fellowships (grant DGE-114747) and W.E.A. by a Fannie & John Hertz Foundation Fellowship. K.D. is supported by the Defense Advanced Research Projects Agency Neuro-FAST program, the National Institute of Mental Health, the National Institute on Drug Abuse, the National Science Foundation, the NOMIS Foundation, the Tarlton Foundation, the Wiegers Family Fund, the Nancy and James Grosfeld Foundation, the H.L. Snyder Medical Foundation, and the Samuel and Betsy Reeves Fund. All tools and methods are distributed and supported freely.

Attached Files

Published - PIIS0896627317303434.pdf

Supplemental Material - mmc1.pdf

Supplemental Material - mmc2.mp4

Accepted Version - nihms923712.pdf


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August 21, 2023
August 21, 2023