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Published September 2, 1988 | public
Journal Article

A Novel Instrument for Separating Large DNA Molecules with Pulsed Homogeneous Electric Fields


A new instrument has been developed for the electrophoretic separation of large DNA molecules that can independently regulate the voltage of each of 24 electrodes and allow the magnitude, orientation, homogeneity, and duration of the electric field to be precisely controlled. Each parameter can be varied at any time during the electrophoretic process. Thus distinct sets of conditions can be combined to optimize the separation of various fragment sizes in a single run. Independent control of electrode voltage allows all of the fields to be generated with electrodes arranged in a closed contour, independent of a particular geometry. This device increases both the resolution in any size range and the speed of separation, especially for DNA molecules larger than 3 megabases.

Additional Information

© 1988 American Association for the Advancement of Science. Received 1 July 1988; accepted 14 July 1988. We thank C. F. Spence for software development, P. Arakelian for technical assistance, B. Jones for preparation of figures, and J. Kobori and R. Kaiser for their critical reading of the manuscript. B.W.B. is an NIH postdoctoral fellow (GM10974). Supported by grants from the NSF (DMB 85-00298) and the Baxter Foundation to L.H.

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August 19, 2023
October 19, 2023