Supporting
information
Figure
S1.
Reverse
phase
HPLC
profiles
for
DNA1
(a)
before
and
(b)
after
1
min
irradiation
with
a
linear
gradient
of
5%–20%
acetonitrile
in
50mM
ammonium
acetate
for
20
min
at
a
flow
rate
of
1.0
ml/min.
Broad
two
peaks
around
5.5
and
7.2
min
come
from
thiol-capping
reagents
(methylmethanethiosulfonate).
Figure
S2.
Reverse
phase
HPLC
profiles
for
DNA1
possessing
one
SH
group
(a)
before
and
(b)
after
10
s
irradiation
in
the
presence
of
oxygen.
Unmodified
DNA
was
used
instead
of
3’-SH-DNA.
Irradiated
sample
contains
10
μ
M
DNA,
100
mM
NaCl,
20
mM
Na
phosphate,
pH
7.0.
(a)
Three
peaks
in
HPLC
profiles
(retention
time:
7.7,
10.6,
and
1
1.3
min)
are
assigned
to
unmodified
DNA,
5’-SH-DNA,
AQ-
DNA,
respectively
.
(b)
After
10
s
irradiation,
new
peaks
appeared
concomitant
with
the
decrease
of
5’-
SH-DNA.
Major
product
of
SH
oxidation
at
8.7
min
represented
by
asterisk
was
characterized
by
MALDI-T
OF
MS
([
M-H]
−
:
calcd
for
5’-SO
3
H-DNA
4496.8:
found
4497,
see
Figure
S3d),
and
assigned
to SO
3
H-DNA.
S1
Figure
S3.
MALDI-T
OF
spectra
for
(a)
SMe-capped
5’-SH-DNA
and
(b)
SMe-capped
3’-SH-DNA
of
DNA1,
(c)
disulfide
DNA
(SS-DNA),
and
(d)
major
oxidation
product
(5’-SO
3
H-DNA)
separated
by
HPLC
after
irradiation.
[M-H]
−
:
calcd
for
SMe-capped
5’-SH-DNA,
SMe-capped
3’-SH-DNA,
SS-
DNA,
and
SO
3
H-DNA,
4494.8,
321
1.0,
7612.8,
and
4496.8,
respectively
.
S2