49
Table S2: qPCR primers
, Related to STAR Methods
sections on
Protein capture, si
RNA
e
xperiments
for
SRP19 and SRP54, and Interferon stimulation experiments
.
target
F primer
R primer
GAPDH
ATTCCACCCATGGCAAATTC
TGGGATTTCCATTGATGACAAG
IFN
-
beta
TTCAGTGTCAGAAGCTCCTGTGG
CTGCT
TAATCTCCTCAGGGATGTCA
ISG54
CTTCCCAGTCTATCATCAACCTT
CCGTCGCTTCTAGCTATGTATCT
18S_1
CAT TCG AAC GTC TGC CCT AT
ACC
CTG
ATT
CCC
CGT
CAC
18S_2
ATT GGA GGG CAA GTC TGG T
CCA
AGA
TCC
AAC
TAC
GAG
CTT
T
LBR
AGT ATA GCC TTC GTC CAA GAA GA
CAA
AGG
TTC
TCA
CTG
CCA
GTT
CALM3
GAC CAT TGA CTT CCC GGA GTT
GAT
GTA
GCC
ATT
CCC
ATC
CTT
G
SRP19
GCCGACCAGGACAGGTTTATT
TCTCTGTAGCTGTAGGATTTTCAAC
SRP54
TGGATTGCAAGGGAGTGGTAA
GGTCAAAAGCCCCTGCTCT
Table S3: Addgene ORFs
, Related to STAR Methods
sections
on
Generation of RNA
binding maps
,
Cloning of expression constructs
.
Gene
Symbol
Synthesized
CDS Na
me
Addgene
clone
number
URL
RRID
ORF1AB
(split
into 16
protein)
NSP1
141255
http://n2t.net/addgene:141255
RRID:Addgene_141255
NSP2
141256
http://n2t.net/addgene:141256
RRID:Addgene_141256
PLPRO
(NSP3)
141257
http://n2t.net/addgene:141257
RRID:Addgene_141257
NSP4
141258
http://n2t.net/addgene:141258
RRID:Addgene_141258
NSP5
1412
59
http://n2t.net/addgene:141259
RRID:Addgene_141259
NSP6
141260
http://n2t.net/addgene:141260
RRID:Addgene_141260
NSP7
141261
http://n2t.net/addgene:141261
RRID:Addgene_141261
NSP8
141262
http://n2t.net/addgene:141262
RRID:Addgene_141262
NSP9
141263
http://n2t.net/addgene:141263
RRID:Addgene_141263
NSP10
141264
http://n2t.net/addgene:141264
RRID:Addgene_141264
NSP11
151991
http://n2t.net/addgene:151991
RRID:Addgene_151991
RNA
-
pol
(NSP12)
141265
http://n2t.net/addgene:141265
RRID:Addgene_141265
Heli (NSP13)
141266
http://n2t.net/addgene:141266
RRID:Addgene_141266
NSP14
141267
http://n2t.net/addgene:141267
RRID:Addgene_141267
NSP15
141268
http://n2t.net/addgene:141268
RRID:Addgene_141268
NSP16
141269
http://n2t.net/addgene:141269
RRID:Addgene_141269
ORF3A
3A
141271
http://n2t.net/addgene:141271
RRID:Addgene_141271
ORF3B
3B
141272
http://n2t.net/addgene:141272
RRID:Addgene_141272
50
E
E
141273
http://n
2t.net/addgene:141273
RRID:Addgene_141273
M
M
141274
http://n2t.net/addgene:141274
RRID:Addgene_141274
ORF6
6
141275
http://n2t.net/addgen
e:141275
RRID:Addgene_141275
ORF7A
7A
141276
http://n2t.net/addgene:141276
RRID:Addgene_141276
ORF7B
7B
141277
http://n2t.net/addgene:1412
77
RRID:Addgene_141277
ORF8
8
141278
http://n2t.net/addgene:141278
RRID:Addgene_141278
ORF9B
9B
141280
http://n2t.net/addgene:141280
RRID
:Addgene_141280
N
N
149330
http://n2t.net/addgene:149330
RRID:Addgene_149330
Table S4: Primers used in mRNA generation
, Related to STAR Methods
sections on
I
n vivo
translation assays
and Lea
der NGFR measurements
.
mRNA
F primer
R primer
template
-
Leader
mCherry
TAATACGACTCACTATAGGCTAGCACC
ATGGTGA
CTACTACTACTTGTA
CAGCTC
GT
Leader mCherry
Gblock
-
Leader
GFP
TAATACGACTCACTATAGGCTAGCACC
ATGGCCCACATCGTGATGGT
CTTGTACAGCTCGTC
CATGCCGA
Spytag
-
eGFP cDNA
+ Leader
mCherry
TAATACGACTCACTATAGATTAAAGGT
TTATACCTTCCCAGG
CTACTACTACTTGTA
CAGCTCGT
Leader mCherry
Gblock
+ Leader
GFP
TAATACG
ACTCACTATAGATTAAAGGT
TTATACCTTCCCAGGTAACAAACCAAC
CAACTTTCGATCTCTTGTAGATCGCACC
ATGGCCCACATCGTGATG
CTTGTACAGCTCGTC
CATGCCGA
Spytag
-
eGFP cDNA
+5
nt
-
SL1
mCherry
TAATACGACTCACTATAGAATTATTAA
AGGTTTATACCTTCCCAGGTAACAAAC
CAACCAACTTTCGATCTCTTGTAGATCG
GCTAGCACCATG
GTGA
CTAC
TACTACTTGTA
CAGCTCGT
Leader mCherry
Gblock
+
SL1
mCherry
TAATACGACTCACTATAGATTAAAGGT
TTATACCTTCCCAGGTAACAAACCGGC
TAGCACCATG
GTGA
CTACTACTACTTGTA
CAGCTCGT
Leader mCherry
Gblock
+SL2
-
SL1
mCherry
TAATACGACTCACTATAGATTAAAGAT
CTCTTGTAGATCAACCAACTTTCGGTTT
ATACCTTCCCAG
GTAACAAACCGGCTA
GCACCATG
GTGA
CTACTACTACTTGTA
CAGCTCGT
Leader mCherry
Gblock
Nsp1
AAAAGGATCCATGGAGAGCCTGGTGCC
AAAACTCGAGTTAGC
CACCGTTCAGTTCAC
Nsp1 mammalian
expression vector
+ Leader
NGFR
ATTAAAGGTTTATACCTTCCCAGGTAA
CAAACCGGCTAGCACCATGGCCACAAC
CATGGA
TTATCGA
TAAGCTTG
GCTGCAG
pB
-
Halo
-
eGFP
-
IRES
-
NGFR
mammalian
expression vector
51
Table S5: Templates used in mRNA generation
, Related to STAR Methods
sections on
I
n
vivo
translation assays
.
Leader
mCherry
Gblock
TCAGTCGCGATCGAACCCTGGAATTCATTAAAGGTTTATACCTTCCCAGGT
AACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTC
TAAACGAACG
GCTAGCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAA
GGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACG
AGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAG
ACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGA
CATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAG
CACCC
CGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG
GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGG
ACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGC
ACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTG
GGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCG
AGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGA
GGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCT
ACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACC
ATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCAT
GGACGAGCTGTACAAGTAGTAGTAGGCGGCCGCATAAAAATTAAAGA
Spytag
-
e
GFP
cDNA
AGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAA
GCTTATACGAAATTAATACGACTCACTATAGGGAGACCACAACGGTTTCCC
GAATTGTGAGCGGATAACAATAGAAATAATTTTGTTTAACTTTAAGAAGG
AGATATATCCATGGCCCACATCGTGATGGTGGACGCCTACAAGCCTACTAA
GGGCAGCGGCTCTAGAATGGCCACAACCATGGTGAGCAAGGGCGA
GGAG
CTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAA
CGGCCACAAGTTCAGCGTGTCTGGCGAGGGCGAGGGCGATGCCACCTACG
GCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCT
GGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCT
ACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAA
GGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAA
GACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCG
AGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAG
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA
GAAGAACGGCATCAAGGCGAACTTCAAGATCCGCCACAACATCGAGGACG
GCAGCG
TGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGAC
GGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTG
AGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGT
GACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGGATTCG
GATCCGAACAAAAACTCATCTCAGAAGAGGATCTGGCGGCCGCTGGATCT
GGTGGCCAGAA
GCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATG
CTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAAGCAGCGAA
GAAAGCGGCTGCAGACGCAAAGAAAAAAGCAGAAGCAGAAGCCGCCAAA
GCCGCAGCCGAAGCGCAGAAAAAAGCCGAGGCAGCCGCTGCGGCACTGA
AGAAGAAAGCGGAAGCGGCAGAAGCAGCTGCAGCTGAAGCAAGAAAGAA
AGCGGCAACTGAAACCGCACAC
CTTACTGGTGTGCGGGGCATCCCGACAT
GGACGTTGAATTATGAATTCACTGGCCGTCGTTTTACA