Rapid preimplantation detection of mutant (shiverer) and normal alleles of the mouse myelin basic protein gene allowing selective implantation and birth of live young
As a model for the detection of human genetic disease in preimplantation embryos, we describe a method in which trophectoderm biopsy samples from viable mouse blastocysts are simultaneously analyzed for the presence of a normal or mutant allele of the myelin basic protein gene by the polymerase chain reaction. The biopsied embryos are kept in culture during analysis of biopsied material and later reintroduced to a foster mother. Prenatal diagnosis can be completed in less than 7 hr. The identity of either amplification product was proved conclusively by direct sequence analysis of amplified products. Ninety-six percent of recovered blastocysts survived biopsy, as judged by re-formation of a blastocyst cavity in culture. Fifty-nine percent of the biopsied embryos established pregnancy by day 6.5, compared to 88% of unmanipulated controls. This approach can be applied to preimplantation diagnosis of human genetic diseases by using extraembryonic cells from blastocysts obtained after in vitro fertilization or uterine lavage. It will make possible the elimination of a mutant allele from a family in a single generation.
Additional Information© 1990 National Academy of Sciences. Contributed by Leroy E. Hood, March 19, 1990. We thank Teresa Geffroy and Eric Furman for their excellent technical assistance, Dr. Melvin Simon for his support, and Drs. Lance Fors, Ray Owen, Debbie Nickerson, Carmie Puckett, and Raul Saavedra for useful criticism. We thank Mark Harmel for his excellent photographic skills. The work was supported by the Markey Foundation, the Whittier Foundation, National Institutes of Health Grant NS14069, and the Medical Research Council U.K. (A.L.M.-H. and D.G.W.).
Published - PNAS-1990-Gomez-4481-4.pdf